Shibata H, Tahira T, Hayashi K
Division of Genome Analysis, Institute of Genetic Information, Kyushu University, Fukuoka, Japan.
Genome Res. 1995 Nov;5(4):400-3. doi: 10.1101/gr.5.4.400.
We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.
我们证明RNA可作为PCR的引物。使用rTth DNA聚合酶至关重要，因为它具有很强的逆转录酶活性。RNA引物可通过体外转录获得，且成本低于化学合成的DNA引物。RNA引发的PCR还开启了在不知道靶核苷酸序列的情况下实现特异性扩增反应的可能性。
