Filler T J, Rickert C H, Zhao B
Institute of Anatomy, Westfälische Wilhelms-University, Münster, Germany.
Anal Quant Cytol Histol. 1995 Dec;17(6):361-5.
For morphologic and functional investigations on the physiology and pathology of human blood platelets, their adhesion plays an important role. This feature is normally assessed concerning qualitative aspects, but there is a lack of quantitative investigations.
We developed a model to quantify in vitro manipulations on thrombocytes by measuring their adhesion areas automatically. This was achieved by combining the VIDAS 2.5 image analysis system with reflection contrast microscopy (RCM). The evaluation was performed independent of the position of the grey value histogram by analyzing each digitized image corresponding to its individual mean grey value.
The applicability and validity were verified from the example of a pharmacologic experiment administering the platelet stimulant oxidized low density lipoprotein (ox-LDL) to thrombocyte suspensions and confirming it by aggregometry. We assessed the temporal increase in adhesion area and mean total adhesion area of activated platelets, comparing unstimulated to stimulated ones. Ox-LDL accelerated the adhesion process and increased the adhesion area.
The combination of RCM with consecutive image analysis provides useful parameters for in vitro examination on vital human platelets. Large amounts of data can be accumulated in a short time for an evaluation of influences of and on blood platelets.
在对人体血小板的生理和病理进行形态学及功能研究时，其黏附起着重要作用。这一特性通常是从定性方面进行评估，但缺乏定量研究。
我们开发了一种模型，通过自动测量血小板的黏附面积来量化对血小板的体外操作。这是通过将VIDAS 2.5图像分析系统与反射对比显微镜（RCM）相结合来实现的。通过分析与每个数字化图像各自的平均灰度值相对应的图像，评估独立于灰度值直方图的位置进行。
以向血小板悬浮液中施用血小板刺激剂氧化型低密度脂蛋白（ox-LDL）的药理实验为例，验证了该模型的适用性和有效性，并通过血小板聚集测定法进行了确认。我们评估了活化血小板黏附面积和平均总黏附面积随时间的增加情况，比较了未刺激血小板和刺激血小板。氧化型低密度脂蛋白加速了黏附过程并增加了黏附面积。
反射对比显微镜与连续图像分析相结合为对有活力的人体血小板进行体外检查提供了有用的参数。可以在短时间内积累大量数据，用于评估对血小板的影响以及血小板所产生的影响。
