Tanaka S, Funakoshi E, Kawahara A, Nemoto K, Fukui T, Suzuki T, Igarashi K, Ichikawa A
Department of Physiological Chemistry, Kyoto University, Japan.
Methods Find Exp Clin Pharmacol. 1995 Nov;17 Suppl C:25-9.
The effect of repeated cold stress (RCS) on both mRNA level and enzyme activity of stomach histidine decarboxylase (HDC) was studied in ddY mice. Following 1-day treatment of RCS, stomach HDC activity, but not its mRNA level, increased two fold. Following 3-day treatment of RCS, which is the essential period for the induction of hyperalgesia in mice, HDC mRNA level and enzyme activity increased in the stomach. After cessation of RCS treatment, HDC mRNA level decreased and reached the level of non-RCS treated mice, but HDC activity did not. The stomach from the 1-day RCS-treated mouse contained proteolytic activity, which converts the in vitro-translated 74 kD HDC species into the 53 kD HDC species. These data demonstrate that RCS-treated mouse stomach induces both the de novo synthesis of the 74 kD HDC species and its proteolytic cleavage to 53 kD HDC species.
在ddY小鼠中研究了反复冷应激（RCS）对胃组织中组氨酸脱羧酶（HDC）的mRNA水平和酶活性的影响。经过1天的RCS处理后，胃HDC活性增加了两倍，但其mRNA水平没有变化。经过3天的RCS处理后（这是诱导小鼠痛觉过敏的关键时期），胃中HDC的mRNA水平和酶活性均增加。停止RCS处理后，HDC的mRNA水平下降并达到未接受RCS处理小鼠的水平，但HDC活性没有下降。经1天RCS处理的小鼠胃中含有蛋白水解活性，可将体外翻译的74kD HDC转化为53kD HDC。这些数据表明，经RCS处理的小鼠胃既诱导了74kD HDC的从头合成，又诱导了其蛋白水解切割为53kD HDC。
