Paul R, Hu B, Musahl C, Hameister H, Knippers R
Division of Biology, Universität Konstanz, Germany.
Cytogenet Cell Genet. 1996;73(4):317-21. doi: 10.1159/000134366.
We have isolated the complete cDNA sequence encoding the human homolog of the yeast replication protein Cdc46/Mcm5. The cDNA was used as a probe for chromosomal fluorescence in situ hybridization (FISH), which localized the human Cdc46 gene (CDC46) to chromosome region 22q13.1-->q13.2. This is the fourth human Mcm gene whose chromosome region has been determined, and it is now apparent that human Mcm genes are widely distributed in the genome. As deduced from the cDNA sequence, human protein hCdc46 is composed of 734 amino acids and contains a central region that is almost 80% identical with the yeast protein. Immunoprecipitation experiments with hCdc46-specific antibodies confirm that essentially all nuclear hCdc46 proteins form a stable dimeric complex with protein P1Mcm3.
我们已经分离出编码酵母复制蛋白Cdc46/Mcm5的人类同源物的完整cDNA序列。该cDNA被用作染色体荧光原位杂交（FISH）的探针，将人类Cdc46基因（CDC46）定位到染色体区域22q13.1→q13.2。这是已确定染色体区域的第四个人类Mcm基因，现在很明显人类Mcm基因在基因组中广泛分布。根据cDNA序列推断，人类蛋白hCdc46由734个氨基酸组成，并且包含一个与酵母蛋白几乎80%相同的中央区域。用hCdc46特异性抗体进行的免疫沉淀实验证实，基本上所有的核hCdc46蛋白都与蛋白P１Mcm3形成稳定的二聚体复合物。
