Dunham W R, Klein S B, Rhodes L M, Marcelo C L
Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.
J Invest Dermatol. 1996 Sep;107(3):332-5. doi: 10.1111/1523-1747.ep12363174.
Keratinocytes were grown in medium with no essential fatty acids as well as in media with specially selected fatty acid augmentations. Gas chromatographic determinations of 21 fatty acids in the phospholipids were correlated with plasma membrane viscosity obtained by electron paramagnetic resonance studies (n = 24). Using standard procedures from multivariate analysis, we derived an expression that modeled the viscosity data as a function of four key fatty acid levels: [formula see text] where the fatty acids are given in mole percent of total lipids and are identified as two number sequences: number of carbons followed by number of double bonds. No other fatty acid made a significant contribution to the regression equation. The range of viscosity was very large, varying from 60 to 120 cP over the sample population. The results are interpreted to indicate that polyunsaturated fatty acids are replaced with monounsaturated fatty acids by the keratinocytes and that dihomogamma-linolenic acid (20:3, n-6) plays an important role in membrane viscosity when essential fatty acids are available in the growth medium of these adult human cultured keratinocytes.
角质形成细胞在不含必需脂肪酸的培养基以及添加了特别选定脂肪酸的培养基中培养。通过气相色谱法测定磷脂中的21种脂肪酸，并将其与通过电子顺磁共振研究获得的质膜粘度相关联（n = 24）。使用多元分析的标准程序，我们得出了一个表达式，该表达式将粘度数据建模为四种关键脂肪酸水平的函数：[公式见原文]，其中脂肪酸以总脂质的摩尔百分比给出，并被识别为两个数字序列：碳原子数后跟双键数。没有其他脂肪酸对回归方程有显著贡献。粘度范围非常大，在样本群体中从60到120厘泊不等。结果表明，角质形成细胞将多不饱和脂肪酸替换为单不饱和脂肪酸，并且在这些成人培养角质形成细胞的生长培养基中有必需脂肪酸时，二高γ-亚麻酸（20:3，n-6）在膜粘度中起重要作用。
