Midis G P, Shen Y, Owen-Schaub L B
Department of Surgical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030-4095, USA.
Cancer Res. 1996 Sep 1;56(17):3870-4.
Fas is a widely expressed membrane-anchored protein that induces apoptosis. Soluble Fas (sFas), generated by alternative mRNA splicing, can antagonize cell-surface Fas function. We have investigated sFas in 104 cancer patients with nonhematopoietic malignancies using a Fas-specific ELISA and immunoprecipitation. Our studies demonstrate an elevated 40-42-kDa sFas species in both patient serum and tumor explants. These observations provide the first evidence that sFas is increased in patients with solid tumors in a manner reflective of disease stage and tumor burden and argue that sFas can be synthesized and released both systemically and locally within the tumor microenvironment.
Fas是一种广泛表达的诱导凋亡的膜锚定蛋白。由可变mRNA剪接产生的可溶性Fas（sFas）可拮抗细胞表面Fas的功能。我们使用Fas特异性酶联免疫吸附测定（ELISA）和免疫沉淀法，对104例非造血系统恶性肿瘤癌症患者的sFas进行了研究。我们的研究表明，患者血清和肿瘤外植体中40 - 42 kDa的sFas种类均有所升高。这些观察结果首次证明，实体瘤患者体内的sFas以反映疾病分期和肿瘤负荷的方式增加，这表明sFas可在肿瘤微环境中全身和局部合成并释放。
