循环血清中可溶性CD95和肿瘤坏死因子-α水平在宫颈癌细胞保护中的临床意义
Clinical Significance of Circulating Serum Levels of sCD95 and TNF-α in Cytoprotection of Cervical Cancer.
作者信息
Agnihotri Saurabh Kumar, Kumar Balawant, Jain Ankita, Anjali Anjali, Negi Mahendra Pal Singh, Sachan Rekha, Bhatt Madan Lal Brahma, Tripathi Raj Kamal, Sachdev Monika
机构信息
Department of Radiotherapy, King George's Medical University, Lucknow 226 003, India.
Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow 226 031, India.
出版信息
Rep Biochem Mol Biol. 2022 Jan;10(4):711-721. doi: 10.52547/rbmb.10.4.711.
BACKGROUND
This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC).
METHODS
Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat cells. Data was scrutinized through statistical analysis.
RESULTS
Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients; their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation. Statistical analysis linked these three parameters for the early screening of CC.
CONCLUSION
Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.
背景
本研究将可溶性CD95（sCD95）和肿瘤坏死因子-α（TNF-α）的血清水平与一种简单的基于细胞的检测方法相关联，以评估血清样本诱导人急性T淋巴细胞白血病细胞（Jurkat细胞）凋亡的能力。可以探索这些参数之间的联系，以设计一种用于宫颈癌（CC）的微创诊断策略。
方法
通过荧光激活细胞分选术（FACS）评估血清样本诱导Jurkat细胞凋亡的情况。采用酶联免疫吸附测定（ELISA）法检测sCD95和TNF-α的血清水平。在与血清孵育的Jurkat细胞中评估c-Jun氨基末端激酶（JNK）磷酸化情况。通过统计分析对数据进行审查。
结果
在CC患者中观察到sCD95的血清水平显著升高，而TNF-α水平降低；他们的血清样本通过降低JNK磷酸化抑制Jurkat细胞凋亡的诱导。统计分析将这三个参数联系起来用于CC的早期筛查。
结论
CC患者中sCD95和TNF-α的血清水平不同，显示出抗凋亡作用，这可用于CC的早期检测。
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