Bishop R F, Bugg H C, Masendycz P J, Lund J S, Gorrell R J, Barnes G L
Department of Gastroenterology, Royal Children's Hospital, Melbourne, Australia.
J Infect Dis. 1996 Sep;174 Suppl 1:S22-9. doi: 10.1093/infdis/174.supplement_1.s22.
Sixty-eight mother-infant pairs were followed for 12-17 months after birth. Rotavirus infections in children were detected by EIA of weekly fecal antigen and anti-rotavirus IgA levels, by EIA of anti-rotavirus IgG in sera at birth, 6, or 12-17 months of age, and by anti-rotavirus EIA IgA and neutralizing antibody (NA) in monthly samples of maternal breast milk. Primary rotavirus infection was detected in 26 children (in 15 [58%] by fecal excretion, 12 [46%] by IgG seroconversion, and 22 [85%] by elevations of IgA anti-rotavirus antibodies [IgA coproconversion] in consecutive fecal specimens). Rotavirus "challenge" was detected by rises in levels of NA in breast milk in 9 (47%) of 19 mothers, including 5 (26%) from pairs in which there was no other evidence of rotavirus infection. Reinfections were detected in 2 children by rotavirus excretion and in 4 by coproconversion. IgA coproconversion is the most sensitive technique for detection of symptomatic and asymptomatic rotavirus infection in young children.
68对母婴在出生后随访了12至17个月。通过每周粪便抗原和抗轮状病毒IgA水平的酶免疫测定（EIA）、出生时、6个月或12至17个月龄时血清中抗轮状病毒IgG的EIA以及母乳每月样本中的抗轮状病毒EIA IgA和中和抗体（NA）来检测儿童的轮状病毒感染。在26名儿童中检测到原发性轮状病毒感染（15名[58%]通过粪便排泄、12名[46%]通过IgG血清转化、22名[85%]通过连续粪便标本中抗轮状病毒抗体IgA升高[IgA粪便转化]）。在19名母亲中的9名（47%）母乳中NA水平升高检测到轮状病毒“激发”，其中5名（26%）来自没有其他轮状病毒感染证据的母婴对。通过轮状病毒排泄在2名儿童中检测到再感染，通过粪便转化在4名儿童中检测到再感染。IgA粪便转化是检测幼儿有症状和无症状轮状病毒感染最敏感的技术。
