Cellerino A, Maffei L, Domenici L
Istituto di Neurofisiologia del CNR, Pisa, Italy.
Eur J Neurosci. 1996 Jun;8(6):1190-7. doi: 10.1111/j.1460-9568.1996.tb01287.x.
We analysed the distribution of brain-derived neurotrophic factor (BNDNF) and its receptor trkB in the adult rat visual cortex, paying particular attention to a GABAergic neuronal subpopulation - the parvalbumin-positive cells. We found expression of trkB in the cell body and apical dendrite of pyramidal neurons and in the cell body of non-pyramidal neurons. Double labelling experiments revealed extensive colocalization of parvalbumin and trkB immunoreactivity in non-pyramidal neurons. Interestingly, the trkB-positive pyramidal neurons appeared surrounded by parvalbumin-labelled boutons. The use of double immunohistochemistry and in situ hybridization histochemistry showed that parvalbumin-positive neurons express trkB mRNA. BDNF mRNA was found in several cells. Coexpression of BDNF mRNA and parvalbumin immunoreactivity was extremely rare. These data strongly suggest that BDNF synthesized by cortical neurons acts as a postsynaptically derived factor for parvalbumin-positive neurons in the adult rat visual cortex.
我们分析了成年大鼠视觉皮层中脑源性神经营养因子（BDNF）及其受体trkB的分布，特别关注了一个γ-氨基丁酸能神经元亚群——小白蛋白阳性细胞。我们发现trkB在锥体神经元的胞体和顶端树突以及非锥体神经元的胞体中表达。双重标记实验显示，在非锥体神经元中，小白蛋白和trkB免疫反应性广泛共定位。有趣的是，trkB阳性锥体神经元似乎被小白蛋白标记的终扣所包围。双重免疫组织化学和原位杂交组织化学的结果表明，小白蛋白阳性神经元表达trkB mRNA。在多个细胞中发现了BDNF mRNA。BDNF mRNA与小白蛋白免疫反应性的共表达极为罕见。这些数据有力地表明，皮层神经元合成的BDNF在成年大鼠视觉皮层中作为小白蛋白阳性神经元的突触后衍生因子发挥作用。
