使用基因打印STR复合试剂盒测定的日本人群中三个STR基因座（TPOX、CSF1PO、THo1）的频率。

Frequency of the three STR loci (TPOX, CSF1PO, THo1), in a Japanese population determined using a Gene Print STR multiplex kit.

作者信息

Yamaguchi H, Takizawa H, Shimasaki C

机构信息

Postgraduate Course of Engineering, Toyama University, Japan.

出版信息

Nihon Hoigaku Zasshi. 1996 Jun;50(3):163-7.

PMID:8752987

Abstract

Allele and genotype frequencies for three tetrametric short tandem repeat loci (TPOX, CSF1PO and TH01) were determined in a Japanese population sample (n = 105) by means of the Gene Print STR Multiplex system PCR kit, electrophoresis of the PCR products in denaturing gels and detection of allelic fragments by silver staining. Six alleles each were found for the TPOX, CSF1PO, and TH01 loci and at the TPOX locus, we identified an additional mutation allele, which consisted of 7 AATG repeats. The TPOX, CSF1PO and TH01 loci were apparently 64, 73, and 74% heterozygous, respectively. All loci met the Hardy-Weinberg criteria using categorized alleles.

摘要

通过基因指纹STR复合扩增系统PCR试剂盒、在变性凝胶中对PCR产物进行电泳以及银染法检测等位基因片段，在一个日本人群样本（n = 105）中测定了三个四核苷酸短串联重复序列位点（TPOX、CSF1PO和TH01）的等位基因和基因型频率。在TPOX、CSF1PO和TH01位点各发现了6个等位基因，并且在TPOX位点，我们鉴定出了一个额外的突变等位基因，它由7个AATG重复序列组成。TPOX、CSF1PO和TH01位点的杂合度分别明显为64%、73%和74%。使用分类后的等位基因，所有位点均符合哈迪-温伯格标准。