Asano K, Taniguchi S, Nakao A, Watanabe T, Kurokawa K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Aug 14;225(2):352-7. doi: 10.1006/bbrc.1996.1179.
Platelet activating factor (PAF) receptor mRNA was semi-quantitated in microdissected rat nephron segments by the reverse transcription-polymerase chain reaction (RT-PCR). PCR primers were designed from two alternative splicing isoforms of rat PAF receptor cDNA; a homologue of human leukocyte PAF receptor cDNA (p172) and the other with a unique sequence in the 5'-noncoding region upstream of the splice acceptor site (p18). Only the expression of mRNA corresponding to p172 was detectable, which was most abundant in the glomerulus, followed in order by: proximal convoluted tubule > proximal straight tubule > outer medullary and cortical collecting duct, distal convoluted tubule > thick ascending limb of Henle's loop. These results suggest the potential physiological roles of PAF in the entire renal tubule as well as in the glomerulus.
采用逆转录聚合酶链反应（RT-PCR）对显微切割的大鼠肾单位各节段中的血小板活化因子（PAF）受体mRNA进行半定量分析。PCR引物是根据大鼠PAF受体cDNA的两种可变剪接异构体设计的；一种与人白细胞PAF受体cDNA同源（p172），另一种在剪接受体位点上游的5'-非编码区具有独特序列（p18）。仅可检测到与p172相对应的mRNA的表达，其在肾小球中最为丰富，依次为：近端曲管＞近端直小管＞外髓和皮质集合管、远端曲管＞亨氏袢粗升支。这些结果提示PAF在整个肾小管以及肾小球中可能具有生理作用。
