Mori M, Aihara M, Shimizu T
Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan.
Invest Ophthalmol Vis Sci. 1997 Nov;38(12):2672-8.
To determine the distribution of platelet-activating factor (PAF) receptor in the rat eye.
Platelet-activating factor receptor messenger RNA (mRNA) expression was evaluated with reverse transcription-polymerase chain reaction (RT-PCR) in RNAs from several rat ocular tissues. Distribution of the expression was determined with in situ hybridization in adult rat eye sections and a 35S-labeled complementary RNA probe synthesized from the rat PAF receptor complementary DNA. In situ hybridization was also done with sections immunostained with OX-42, a microglia marker.
RT-PCR revealed that levels of PAF receptor expression were similar among the ocular tissues studied. The in situ hybridization signals were found in the corneal epithelium, iris and ciliary body, and ganglion and microglial cells in the retina.
Platelet-activating factor receptor mRNA was ubiquitously expressed in the rat eye with relative concentration in the corneal epithelium, iris and ciliary body, and retinal ganglion and microglial cells.
确定血小板活化因子（PAF）受体在大鼠眼中的分布。
采用逆转录-聚合酶链反应（RT-PCR）评估几种大鼠眼组织RNA中血小板活化因子受体信使核糖核酸（mRNA）的表达。用成年大鼠眼切片原位杂交和从大鼠PAF受体互补DNA合成的35S标记互补RNA探针确定表达的分布。还用小胶质细胞标志物OX-42免疫染色的切片进行原位杂交。
RT-PCR显示，在所研究的眼组织中，PAF受体表达水平相似。原位杂交信号见于角膜上皮、虹膜和睫状体以及视网膜中的神经节和小胶质细胞。
血小板活化因子受体mRNA在大鼠眼中普遍表达，在角膜上皮、虹膜和睫状体以及视网膜神经节和小胶质细胞中相对集中。
