Kawamura-Konishi Y, Neya S, Funasaki N, Suzuki H
Department of Biosciences, School of Science, Kitasata University, Kanagawa, Japan.
Biochem Biophys Res Commun. 1996 Aug 14;225(2):537-44. doi: 10.1006/bbrc.1996.1208.
The catalytic antibody which catalyzes insertion of a cupric ion into mesoporphyrin bound ferric N-methyl mesoporhyrin and ferric mesoporhyrin to yield the respective complexes. The binding affinity of cyanide to the ferric iron in the complexes was compared with that of free ferric porphyrins. The results indicate that the one side of the porphyrin plane in the complex is thoroughly exposed to surrounding solvents and the other side interacts with the protein molecule. The cyanide binding suggests the specific orientation of distorted porphyrin at the binding site of the antibody.
催化抗体可催化将铜离子插入与铁N-甲基中卟啉和铁中卟啉结合的中卟啉中，以产生相应的配合物。将配合物中氰化物与铁离子的结合亲和力与游离铁卟啉的结合亲和力进行了比较。结果表明，配合物中卟啉平面的一侧完全暴露于周围溶剂中，另一侧与蛋白质分子相互作用。氰化物结合表明扭曲的卟啉在抗体结合位点的特定取向。
