Cochran A G, Schultz P G
Department of Chemistry, University of California, Berkeley 94720.
Science. 1990 Aug 17;249(4970):781-3. doi: 10.1126/science.2389144.
An antibody elicited to a distorted N-methyl porphyrin catalyzed metal ion chelation by the planar porphyrin. At fixed Zn2+ and Cu2+ concentrations, the antibody-catalyzed reaction showed saturation kinetics with respect to the substrate mesoporphyrin IX (2) and was inhibited by the hapten, N-methylmesoporphyrin IX (1). The turnover number of 80 hour-1 for antibody-catalyzed metallation of 2 with Zn2+ compares with an estimated value of 800 hour-1 for ferrochelatase. The antibody also catalyzed the insertion of Co2+ and Mn2+ into 2, but it did not catalyze the metallation of protoporphyrin IX (3) or deuteroporphyrin IX (4). The antibody has high affinity for several metalloporphyrins, suggesting an approach to developing antibody-heme catalysts for redox or electron transfer reactions.
一种针对扭曲的N-甲基卟啉产生的抗体，可催化平面卟啉的金属离子螯合反应。在固定的Zn2+和Cu2+浓度下，抗体催化反应对底物中卟啉IX（2）呈现饱和动力学，且被半抗原N-甲基中卟啉IX（1）抑制。抗体催化2与Zn2+进行金属化反应的周转数为80 h-1，而铁螯合酶的估计周转数为800 h-1。该抗体还催化Co2+和Mn2+插入到2中，但不催化原卟啉IX（3）或脱氧卟啉IX（4）的金属化反应。该抗体对几种金属卟啉具有高亲和力，这为开发用于氧化还原或电子转移反应的抗体-血红素催化剂提供了一种途径。
