Loseva E F, Kolomiets O L, Il'inskaia L E, Bashkirov V I
Genetika. 1996 Apr;32(4):523-31.
Earlier, polyclonal antibodies to Escherichia coli RecA protein were used to identify immunologically related proteins in meiotic spermatocytes of different eukaryotes. At least one such protein proved to be a component of the synaptonemal complex (SC) [1]. Subsequent experiments on localization of RecA-like antigens in SCs of spermatocytes were performed by indirect immunocytochemical methods and electron microscopy, which showed that RecA-like protein(s) at early leptotene are largely associated with chromatin. During SC formation (at leptotene and zygotene), they are found in both lateral elements and the central space of SC. In some cases, RecA-like proteins are associated with SC substructures that resemble recombination nodules. When spermatocytes enter late diplotene, Rec-A-like proteins cease to be detected in SC structures.
早期，针对大肠杆菌RecA蛋白的多克隆抗体被用于鉴定不同真核生物减数分裂精子细胞中的免疫相关蛋白。至少有一种这样的蛋白被证明是联会复合体（SC）的一个组成部分[1]。随后通过间接免疫细胞化学方法和电子显微镜对精子细胞SC中RecA样抗原进行定位实验，结果表明，早期细线期的RecA样蛋白主要与染色质相关。在SC形成过程中（细线期和偶线期），它们存在于SC的侧生元件和中央空间中。在某些情况下，RecA样蛋白与类似于重组节的SC亚结构相关。当精子细胞进入双线期末期时，在SC结构中不再检测到RecA样蛋白。
