[Role of free radical oxidation in the pathogenesis of acute hepatic necrosis in visceral-ischemic shock].

The mechanisms responsible for necrotic damage to liver cells at the stages of experimental visceral-ischemic shock were studied by computerized morphometric techniques for interphasic nuclei of hepatocytes. A number of geometric, optical, and textural parameters of the structure of chromatin have been defined, which characterize the extent of its damage as shock progresses. The increment of degenerative signs of the nuclear structures occurred concurrently with the enhancement of free radical processes, which may be indicative of the fact that there is their pathophysiological relationship. The findings were comparable with those of a multiparametric analysis of chromatin in the isolated hepatocytes exposed to free radical oxidation. The coincidence of temporal and structural parameters of hepatocytic nuclear alteration in shock and in the model system makes the authors conclude that in visceral-ischemic shock a great role in the genesis of cytolysis is played by the generation of active oxygen forms and their direct or lipid peroxidation-mediated action on the nuclear structures.