Dimster-Denk D, Rine J
Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA.
Mol Cell Biol. 1996 Aug;16(8):3981-9. doi: 10.1128/MCB.16.8.3981.
Sterols and all nonsterol isoprenoids are derived from the highly conserved mevalonate pathway. In animal cells, this pathway is regulated in part at the transcriptional level through the action of sterol response element-binding proteins acting at specific DNA sequences near promoters. Here we extend at least part of this regulatory paradigm to the ERG10 gene, which encodes a sterol-biosynthetic enzyme of Saccharomyces cerevisiae. Specifically, the discovery of sterol-mediated feedback control of ERG10 transcription is reported. Deletion analysis of the ERG10 promoter region identified sequences involved in the expression of ERG10. This regulatory axis appeared to involve sterol levels, as a late block in the pathway that depletes sterol, but not nonsterol isoprenoids, was able to elicit the regulatory response.
甾醇和所有非甾醇类异戊二烯均源自高度保守的甲羟戊酸途径。在动物细胞中，该途径部分受甾醇反应元件结合蛋白作用于启动子附近特定DNA序列的转录水平调控。在此，我们将这种调控模式至少部分扩展至ERG10基因，该基因编码酿酒酵母的一种甾醇生物合成酶。具体而言，报道了甾醇介导的ERG10转录反馈控制的发现。对ERG10启动子区域的缺失分析确定了参与ERG10表达的序列。该调控轴似乎涉及甾醇水平，因为途径中消耗甾醇而非非甾醇类异戊二烯的晚期阻断能够引发调控反应。
