Chande C, Thakar Y S, Pande S, Dhanvijay A G, Shrikhande A V, Saoji A M
Department of Microbiology, Government Medical College, Nagpur.
Indian J Pathol Microbiol. 1996 Jan;39(1):27-32.
The present study was planned to develop an immunization protocol to decide the bleeding modalities for harvesting anti IgG antibody from the immunized rabbit. A fourteen dose immunization protocol (four primary and ten boosters) of the purified human IgG spread over the one calendar year was executed. The antibody titre estimated by Reverse Single Radial Immunodiffusion displayed a six-phased pattern. The titre following the initial immunization ranged between nil to 1.02, characteristic of the primary response while the titres after 7th and 8th boosters (phase V) ranged between 0.5 to 3.87 consistent with secondary response. Phase II, III, IV had moderately elevated titres. The antibody titre amongst the six phases reached to its peak generally by the 12th day after the last dose of protocol and it took about 60 days to reach to its basal level. Administration of antigen with the higher levels of residual antibody did not produce high titre antibody and is probably ascribed to elimination of antigen through an immunecomplex mechanism. Based on the data we recommend that 15 batches. (3 per phase, phase I to phase V) with a total yield of 100 to 120 ml of serum can be procured from one immunised animal over the span of one calendar year and that should make the programme cost effective.
本研究旨在制定一种免疫方案，以确定从免疫兔中采集抗IgG抗体的采血方式。执行了一项为期一年的纯化人IgG的十四剂免疫方案（四剂初次免疫和十剂加强免疫）。通过反向单向放射免疫扩散估计的抗体滴度呈现出六阶段模式。初次免疫后的滴度在零至1.02之间，这是初次反应的特征，而第7次和第8次加强免疫后（第五阶段）的滴度在0.5至3.87之间，与二次反应一致。第二、三、四阶段的滴度适度升高。六个阶段中的抗体滴度通常在方案最后一剂后的第12天达到峰值，大约需要60天才能降至基础水平。使用残留抗体水平较高的抗原进行给药不会产生高滴度抗体，这可能归因于通过免疫复合物机制清除抗原。根据这些数据，我们建议在一个日历年的时间内，从一只免疫动物身上可以采集15批（第一阶段至第五阶段各3批），血清总产量为100至120毫升，这将使该方案具有成本效益。
