Thakar Y S, Kulkarni C, Pande S, Dhanvijay A G, Shrikhande A V, Saoji A M
Department of Microbiology, Government Medical College, Nagpur, India.
Indian J Exp Biol. 1993 May;31(5):426-9.
Reverse Single Radial Immunodiffusion (SRID) for estimating titre of anti IgG antisera is reported. Unlike the conventional radial immunodiffusion, the antigen (IgG) is held immobile in the gel while the antibody (Anti IgG) diffuses radially from the well (7 microliters) and the diameter of the resulting immuneprecipitates after immunodiffusion at 4 degrees C for 24 hr, represents a linear correlation with the antibody titre. The procedure was standardised by an extensive trial and error employing different concentrations of human IgG in the gel (60-240 micrograms) against varying dilutions of the standard antibody (titre: 3.8 mg/ml). The best results were obtained at 80 micrograms of IgG in the gel. The locally raised rabbit anti IgG antisera displayed a distinctive titre pattern under optimised conditions. Technical reproducibility, high-sensitivity threshold (0.25 mg/ml), simultaneous visual scrutiny of several antibody batches at a glance and ability to assess the shelf life of the stored antisera are its distinct assets.
本文报道了用于估计抗IgG抗血清效价的反向单扩散免疫比浊法（SRID）。与传统的免疫比浊法不同，抗原（IgG）固定在凝胶中，而抗体（抗IgG）从孔（7微升）呈放射状扩散，在4℃免疫扩散24小时后产生的免疫沉淀直径与抗体效价呈线性相关。通过在凝胶中使用不同浓度的人IgG（60 - 240微克）对抗标准抗体的不同稀释度（效价：3.8毫克/毫升）进行广泛的反复试验，对该方法进行了标准化。在凝胶中80微克IgG时获得了最佳结果。在优化条件下，本地制备的兔抗IgG抗血清呈现出独特的效价模式。技术重现性、高灵敏度阈值（0.25毫克/毫升）、能够一眼同时目视检查多个抗体批次以及评估储存抗血清的保质期是其独特优势。
