Zhong W, Feder J N, Jiang M M, Jan L Y, Jan Y N
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724, USA.
Neuron. 1996 Jul;17(1):43-53. doi: 10.1016/s0896-6273(00)80279-2.
During Drosophila neurogenesis, differential segregation of Numb is necessary for daughter cells of asymmetric divisions to adopt distinct fates, at least partly by biasing the Notch-mediated cell-cell interaction. We have isolated a highly conserved mammalian homolog of Drosophila numb, m-numb. During mouse cortical neurogenesis, m-Numb is asymmetrically localized to the apical membrane of dividing ventricular neural progenitors. Depending upon the orientation of the cleavage plane, m-Numb may be distributed into one or both of the daughter cells. When expressed in Drosophila embryos, m-Numb is localized asymmetrically in dividing neural precursors and rescues the numb mutant phenotype. Furthermore, m-Numb can physically interact with mouse Notch1. We propose that some shared molecular mechanisms, both cell-intrinsic and cell-extrinsic, generate asymmetric cell divisions during neurogenesis of vertebrates and invertebrates.
在果蝇神经发生过程中，Numb的差异分离对于不对称分裂的子细胞采用不同命运是必要的，至少部分是通过偏向Notch介导的细胞间相互作用来实现的。我们分离出了果蝇numb的一个高度保守的哺乳动物同源物m-numb。在小鼠皮质神经发生过程中，m-Numb不对称地定位于分裂的脑室神经祖细胞的顶端膜。根据分裂平面的方向，m-Numb可能分布到一个或两个子细胞中。当在果蝇胚胎中表达时，m-Numb不对称地定位于分裂的神经前体细胞中，并挽救了numb突变体表型。此外，m-Numb可以与小鼠Notch1发生物理相互作用。我们提出，一些共同的分子机制，包括细胞内在和细胞外在机制，在脊椎动物和无脊椎动物的神经发生过程中产生不对称细胞分裂。
