酿酒酵母细胞壁中热休克蛋白70（Hsp70）家族的蛋白质成员。
Members of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae.
作者信息
López-Ribot J L, Chaffin W L
机构信息
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, 79430, USA.
出版信息
J Bacteriol. 1996 Aug;178(15):4724-6. doi: 10.1128/jb.178.15.4724-4726.1996.
Abstract
Western blot (immunoblot) analysis of cell wall and cytosolic extracts obtained from parental and ssa1 and ssa2 single- and double-mutant strains of Saccharomyces cerevisiae showed that the heat shock protein 70 (Hsp70) products of these genes, previously thought to be restricted to the cell interior, are also present in the cell wall. A cell wall location was further confirmed by indirect immunofluorescence with intact cells and biotinylation of extracellular Hsp70. Hsp70s have been implicated in translocation across the membrane and as molecular chaperones, and changes in the profile of cell wall proteins suggested that these proteins may have a similar role in the cell wall.
摘要
对酿酒酵母亲本、ssa1和ssa2单突变体及双突变体菌株的细胞壁和胞质提取物进行蛋白质免疫印迹分析，结果显示这些基因的热休克蛋白70（Hsp70）产物，此前认为仅存在于细胞内部，也存在于细胞壁中。完整细胞的间接免疫荧光和细胞外Hsp70的生物素化进一步证实了其在细胞壁中的定位。Hsp70s参与跨膜转运并作为分子伴侣，细胞壁蛋白质谱的变化表明这些蛋白质在细胞壁中可能具有类似作用。
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