Songok E M, Tukei P M, Mulaa F J
Virus Research Centre, Kenya Medical Research Institute, Nairobi, Kenya.
East Afr Med J. 1996 Feb;73(2):88-90.
In a bid to determine the HIV-1 subtype variants in transmission in Nairobi and its possible association with clinical status, we screened 207 confirmed HIV-1 positive patients visiting HIV/AIDS laboratory at the Virus Research Centre in Nairobi between January and March 1994. We used a selfmade ELISA obtained from an established panel of HIV-1 V3 loop peptides (ANRS, France) and derived from seven isolates: MN, HXB2, SC, Z6, Z2, ELI and CDC4. Test samples were obtained from 95 blood donors and medical examination attendees, 57 patients with chronic diarrhoea, 31 confirmed pulmonary tuberculosis, 16 with pneumonia and 12 herpes zoster. Out of the total, 21.5% had antibodies against the MN strain, 19.1% had against the Z2 strain while reaction against the HXB2 strain was observed in 17.2%. SC, CDC4, Z6 and ELI had prevalences of 11.5%, 6.2%, 5.3% and 3.8% respectively. Fifteen per cent of the tested sera showed no reaction to any of the used peptides. Strong and significant associations were observed between the total number of strains a sample react to and the clinical state. We infer that both the North American consensus strains (MN and HXB2) and the African isolates (Z2 and Z6) are predominant in Nairobi. The correlation between antibody reactivity and clinical state is an interesting observation that necessitates an expanded study and, the use of strain specific peptides maybe a sensitive and easier method for use for molecular epidemiological purposes.
为了确定内罗毕地区HIV-1传播中的亚型变体及其与临床状况的可能关联，我们对1994年1月至3月期间前往内罗毕病毒研究中心的HIV/AIDS实验室就诊的207名确诊HIV-1阳性患者进行了筛查。我们使用了一种自制的ELISA试剂盒，该试剂盒由一组既定的HIV-1 V3环肽（法国国家艾滋病研究与预防机构）制备，来源于7株病毒分离株：MN、HXB2、SC、Z6、Z2、ELI和CDC4。检测样本来自95名献血者和体检者、57名慢性腹泻患者、31名确诊肺结核患者、16名肺炎患者和12名带状疱疹患者。在所有样本中，21.5%的样本对MN毒株有抗体，19.1%的样本对Z2毒株有抗体，而对HXB2毒株有反应的样本占17.2%。SC、CDC4、Z6和ELI的流行率分别为11.5%、6.2%、5.3%和3.8%。15%的检测血清对任何一种使用的肽都没有反应。样本对毒株的反应总数与临床状态之间存在强烈且显著的关联。我们推断，北美共识毒株（MN和HXB2）以及非洲分离株（Z2和Z6）在内罗毕占主导地位。抗体反应性与临床状态之间的相关性是一个有趣的观察结果，需要进行更广泛的研究，并且使用毒株特异性肽可能是一种用于分子流行病学目的的敏感且简便的方法。
