Di Como C J, Arndt K T
Cold Spring Harbor Laboratory, New York 11724, USA.
Genes Dev. 1996 Aug 1;10(15):1904-16. doi: 10.1101/gad.10.15.1904.
We identified an essential Saccharomyces cerevisiae protein, Tap42, that associates with Sit4, a type 2A-related protein phosphatase, and with the type 2A phosphatase catalytic subunits. The association of Tap42 with the phosphatases does not require the previously identified phosphatase subunits. Genetic analysis suggests that Tap42 functions positively with both phosphatases. Mutations in TAP42 can confer almost complete rapamycin resistance. In addition, Tap42/Sit4 and Tap42/PP2A complex formation is regulated by nutrient growth signals and the rapamycin-sensitive Tor signaling pathway. These findings, combined with the defect in translation of the tap42-11 mutant at the nonpermissive temperature, suggest that Tap42, Sit4, and PP2A are components of the Tor signaling pathway.
我们鉴定出一种酿酒酵母必需蛋白Tap42，它与2A型相关蛋白磷酸酶Sit4以及2A型磷酸酶催化亚基相关联。Tap42与磷酸酶的关联并不需要先前鉴定出的磷酸酶亚基。遗传分析表明Tap42与这两种磷酸酶均发挥正向功能。TAP42中的突变可赋予几乎完全的雷帕霉素抗性。此外，Tap42/Sit4和Tap42/PP2A复合物的形成受营养生长信号和雷帕霉素敏感的Tor信号通路调控。这些发现，结合tap42 - 11突变体在非允许温度下翻译缺陷，表明Tap42、Sit4和PP2A是Tor信号通路的组成部分。
