French R A, Zachary J F, Dantzer R, Frawley L S, Chizzonite R, Parnet P, Kelley K W
Department of Veterinary Pathobiology, University of Illinois, Urbana-Champaign 61801, USA.
Endocrinology. 1996 Sep;137(9):4027-36. doi: 10.1210/endo.137.9.8756580.
Interleukin-1 alpha (IL-1 alpha) and IL-1 beta bind to either the p80 type I IL-1 receptor (IL-1RI) or the p68 type II IL-1R (IL-1RII) on both T and B lymphocytes. We and others have previously shown that the anterior pituitary gland also has specific high affinity binding sites for IL-1 alpha (Kd = approximately 1 nM) and expresses transcripts for both isoforms of the IL-1R. However, the identity of cells in the anterior pituitary gland that express the IL-1R and whether different populations of adenohypophyseal cells express different isoforms of the IL-1R remain unknown. Here we have used a combination of immunohistochemistry and histochemistry to localize IL-1RI and IL-1RII to specific target cells in the mouse anterior pituitary gland. Perfusion-fixed, paraffin-embedded sections of anterior pituitary gland were immunolabeled with well characterized monoclonal antibodies to either IL-1RI or IL-1RII and counterstained using a modified Gomori's method to document acidophils and basophils. Immunolabeling demonstrated that both IL-1RI and IL-1RII were abundantly expressed on a single population of anterior pituitary gland cells and that these cells could be classified on the basis of histochemical staining as a subpopulation of acidophils. The distribution, morphology, and proportion of cells immunolabeled for IL-1RI and IL-1RII were consistent with GH-synthesizing cells. To confirm this hypothesis, a modified indirect avidin-biotin complex, sequential peroxidase/alkaline phosphatase technique was used to label anterior pituitary gland cells with antibodies to IL-1RI followed by antibodies to IL-1RII, GH, PRL, or ACTH. The IL-1RI-positive cells predominately coexpressed IL-1RII and GH, but very little, if any, PRL or ACTH. These data establish that the predominant cell population in the murine anterior pituitary gland that constitutively expresses IL-1R stain as acidophils histochemically, is round to oval with dense granular cytoplasm and eccentric nuclei, synthesizes GH, and simultaneously expresses IL-1RI and IL-1RII isoforms.
白细胞介素 -1α（IL -1α）和白细胞介素 -1β可与T淋巴细胞和B淋巴细胞上的p80 I型白细胞介素 -1受体（IL -1RI）或p68 II型白细胞介素 -1受体（IL -1RII）结合。我们和其他人之前已经表明，垂体前叶也有针对IL -1α的特异性高亲和力结合位点（解离常数Kd约为1 nM），并且表达IL -1R两种亚型的转录本。然而，垂体前叶中表达IL -1R的细胞身份以及腺垂体细胞的不同群体是否表达不同亚型的IL -1R仍然未知。在这里，我们使用免疫组织化学和组织化学相结合的方法，将IL -1RI和IL -1RII定位到小鼠垂体前叶的特定靶细胞。垂体前叶经灌注固定、石蜡包埋的切片用针对IL -1RI或IL -1RII的特征明确的单克隆抗体进行免疫标记，并用改良的Gomori方法进行复染以记录嗜酸性粒细胞和嗜碱性粒细胞。免疫标记显示，IL -1RI和IL -1RII在垂体前叶的单一细胞群体中大量表达，并且这些细胞可以根据组织化学染色分类为嗜酸性粒细胞的一个亚群。针对IL -1RI和IL -1RII进行免疫标记的细胞的分布、形态和比例与生长激素合成细胞一致。为了证实这一假设，使用改良的间接抗生物素蛋白 - 生物素复合物、顺序过氧化物酶/碱性磷酸酶技术，先用抗IL -1RI抗体标记垂体前叶细胞，然后用抗IL -1RII、生长激素、催乳素或促肾上腺皮质激素抗体进行标记。IL -1RI阳性细胞主要共表达IL -1RII和生长激素，但很少（如果有的话）共表达催乳素或促肾上腺皮质激素。这些数据表明，小鼠垂体前叶中组成性表达IL -1R的主要细胞群体在组织化学上染色为嗜酸性粒细胞，呈圆形至椭圆形，细胞质致密且有颗粒，细胞核偏心，合成生长激素，同时表达IL -1RI和IL -1RII亚型。
