Capellades M, Torres M A, Bastisch I, Stiefel V, Vignols F, Bruce W B, Peterson D, Puigdomènech P, Rigau J
Departament de Genètica Molecular, CID-CSIC, Barcelona, Spain.
Plant Mol Biol. 1996 May;31(2):307-22. doi: 10.1007/BF00021792.
The pattern of expression directed by the promoter of the maize caffeic acid O-methyltransferase (COMT) gene was studied by histochemical and fluorometric beta-glucuronidase (GUS) analysis in transgenic maize and tobacco plants. The COMT promoter directs GUS expression to the xylem and the other tissues undergoing lignification, and it responds to wounding and to elicitors. In transgenic maize plants, expression of GUS corresponds to the pattern of expression of the endogenous COMT gene as determined by northern analysis and in situ hybridization. The pattern in transgenic tobacco plants clearly shows that the maize promoter sequence is recognized by tobacco transcriptional factors, in spite of the anatomical differences and the evolutionary distance between these two species. The results suggest that the most significant promoter signals that induce the specific expression of the lignin COMT are conserved in different species.
通过组织化学和荧光β-葡萄糖醛酸酶（GUS）分析，在转基因玉米和烟草植株中研究了玉米咖啡酸O-甲基转移酶（COMT）基因启动子指导的表达模式。COMT启动子将GUS表达导向木质部和其他正在进行木质化的组织，并且它对创伤和诱导子有反应。在转基因玉米植株中，GUS的表达与通过Northern分析和原位杂交确定的内源性COMT基因的表达模式相对应。转基因烟草植株中的模式清楚地表明，尽管这两个物种在解剖结构上存在差异且进化距离较远，但玉米启动子序列仍能被烟草转录因子识别。结果表明，诱导木质素COMT特异性表达的最重要启动子信号在不同物种中是保守的。
