Roberts C W, Roberts J W
Section of Biochemistry, Molecular and Cell Biology Cornell University Ithaca, New York 14853, USA.
Cell. 1996 Aug 9;86(3):495-501. doi: 10.1016/s0092-8674(00)80122-1.
RNA polymerase recognizes its promoters through base-specific interaction between defined segments of DNA and the sigma subunit of the enzyme. This interaction leads to separation of base pairs and exposure of the template strand for RNA synthesis. We show that base-specific recognition by the sigma 70 holoenzyme in this process involves primarily nontemplate strand bases in the -10 promoter region. We suggest that melting involves the persistence of these contacts as the bound duplex (closed) form is converted to the single-stranded (open) form of the enzyme-promoter complex.
RNA聚合酶通过DNA特定片段与该酶的σ亚基之间的碱基特异性相互作用来识别其启动子。这种相互作用导致碱基对分离，并使模板链暴露以进行RNA合成。我们发现，在此过程中，σ70全酶的碱基特异性识别主要涉及-10启动子区域中的非模板链碱基。我们认为，解链涉及这些接触的持续存在，因为结合的双链体（闭合）形式转变为酶-启动子复合物的单链（开放）形式。
