Elloso M M, van der Heyde H C, Troutt A, Manning D D, Weidanz W P
Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706, USA.
J Immunol. 1996 Sep 1;157(5):2096-102.
We examined the cellular and molecular basis of the proliferative response of human gamma delta T cells in cultures of PBMC stimulated with blood-stage Plasmodium falciparum malarial Ag. Flow cytometry revealed that maximal gamma delta T cell proliferation occurs after maximal CD4+ alpha beta T cell proliferation. Depletion of CD4+ T cells from PBMC before stimulation with malarial Ag markedly reduces the number of proliferating gamma delta T cells, which suggests that CD4+ T cells function in providing help to gamma delta T cells to respond to this parasite Ag. Removal of gamma delta T cells, however, did not alter the expansion of the CD4+ T cell subset. The addition of exogenous IL-2, IL-4, or IL-15 restored the capacity of gamma delta T cells to proliferate in Ag-stimulated cultures of PBMC depleted of CD4+ T cells. mAbs specific for the alpha- and beta-subunits of the IL-2 receptor inhibit the gamma delta T cell subset expansion in cultures stimulated with malarial Ag. Taken together, these findings suggest that the proliferation of gamma delta T cells in response to malarial Ag is dependent on the presence of CD4+ alpha beta T cells, but the requirement for CD4+ alpha beta T cells can be met by cytokines that use the IL-2R.
我们研究了在恶性疟原虫血期抗原刺激的外周血单个核细胞（PBMC）培养物中，人γδT细胞增殖反应的细胞和分子基础。流式细胞术显示，γδT细胞的最大增殖发生在CD4⁺αβT细胞最大增殖之后。在用疟原虫抗原刺激之前，从PBMC中去除CD4⁺T细胞显著减少了增殖的γδT细胞数量，这表明CD4⁺T细胞在为γδT细胞提供帮助以响应这种寄生虫抗原方面发挥作用。然而，去除γδT细胞并没有改变CD4⁺T细胞亚群的扩增。添加外源性白细胞介素-2（IL-2）、白细胞介素-4（IL-4）或白细胞介素-15可恢复γδT细胞在去除CD4⁺T细胞的PBMC抗原刺激培养物中的增殖能力。针对IL-2受体α和β亚基的单克隆抗体抑制了疟原虫抗原刺激培养物中γδT细胞亚群的扩增。综上所述，这些发现表明，γδT细胞对疟原虫抗原的增殖依赖于CD4⁺αβT细胞的存在，但使用IL-2R的细胞因子可以满足对CD4⁺αβT细胞的需求。
