Denner J, Persin C, Vogel T, Haustein D, Norley S, Kurth R
Paul-Ehrlich-Institute, Langen/Frankfurt, Germany.
J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Aug 15;12(5):442-50. doi: 10.1097/00042560-199608150-00002.
The transmembrane glycoproteins of all retroviruses contain a conserved region composed of a leucine zipper, an immunosuppressive domain, and an immunodominant Cys-Cys loop. The amino acid sequence of the immunosuppressive domain of gp41 of human immunodeficiency virus type 1 (HIV-1; amino acids 583-599) is closely related, but not identical, to the immunosuppressive domains of type C and D retroviruses. A synthetic peptide corresponding to the immunosuppressive domain of HIV-1 (immunosuppressive peptide, ISU-peptide) inhibits mitogen and lymphokine stimulation of T lymphocytes. It is interspecies reactive and inhibits both human and mouse lymphocytes. The inhibitory effect is not based on direct cytotoxicity and the peptide is immunosuppressive only when conjugated to a carrier protein. The ISU-peptide of HIV-1 also inhibits B lymphocyte stimulation by the B cell mitogen lipopolysaccharide and by specific antibodies against delta and mu chains of cell surface immunoglobulins. These data suggest that the immunosuppressive domain of gp41 may play an important role in the immunopathogenesis of AIDS.
所有逆转录病毒的跨膜糖蛋白都含有一个保守区域，该区域由亮氨酸拉链、免疫抑制结构域和免疫显性的半胱氨酸-半胱氨酸环组成。1型人类免疫缺陷病毒（HIV-1；氨基酸583-599）的gp41免疫抑制结构域的氨基酸序列与C型和D型逆转录病毒的免疫抑制结构域密切相关，但并不相同。一种与HIV-1免疫抑制结构域相对应的合成肽（免疫抑制肽，ISU-肽）可抑制T淋巴细胞的丝裂原和淋巴因子刺激。它具有种间反应性，可抑制人类和小鼠淋巴细胞。这种抑制作用并非基于直接细胞毒性，且该肽只有在与载体蛋白偶联时才具有免疫抑制作用。HIV-1的ISU-肽还可抑制B细胞丝裂原脂多糖以及针对细胞表面免疫球蛋白δ链和μ链的特异性抗体对B淋巴细胞的刺激。这些数据表明，gp41的免疫抑制结构域可能在艾滋病的免疫发病机制中起重要作用。
