分辨率为2.5埃的CAP-DNA复合物结构：蛋白质-DNA界面的完整图景。

Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface.

作者信息

Parkinson G, Wilson C, Gunasekera A, Ebright Y W, Ebright R H, Berman H M

机构信息

Department of Chemistry and The Waksman Institute, Rutgers University, New Brunswick, NJ 08855-0939, USA.

出版信息

J Mol Biol. 1996 Jul 19;260(3):395-408. doi: 10.1006/jmbi.1996.0409.

DOI:10.1006/jmbi.1996.0409

PMID:8757802

Abstract

The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.

摘要

之前已经报道了分辨率为3.0埃的CAP-DNA复合物的晶体结构。由于技术原因，所报道的结构是使用一个缺少对CAP-DNA相互作用至关重要的两个磷酸基团的缺口DNA分子确定的。在这项工作中，我们报道了使用一个具有对CAP-DNA相互作用至关重要的所有磷酸基团的DNA分子，分辨率为2.5埃的CAP-DNA复合物的晶体结构。目前的分辨率能够明确鉴定CAP-DNA复合物中氨基酸-碱基和氨基酸-磷酸基团之间的氢键接触。此外，目前的分辨率能够准确界定CAP-DNA复合物中弯曲DNA的构象。