Chenthamarakshan V, Reddy M V, Harinath B C
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.
Trans R Soc Trop Med Hyg. 1996 May-Jun;90(3):252-4. doi: 10.1016/s0035-9203(96)90236-9.
Due to the non-availability of sufficient parasite material from Wuchereria bancrofti, a heterologous filarial antigen from Brugia malayi has been investigated for the diagnosis of bancroftian filariasis. B. malayi microfilarial excretory/secretory antigen (BmmfES) effectively inhibited the binding of circulating filarial antigen fractions (CFA2-1, 9, 11 and 12) from microfilaraemic cases, and of W. bancrofti microfilarial excretory/secretory antigen, to the immunoglobulin G (IgG) fraction of anti-filarial serum immunoglobulins. BmmfES was separated by ion-exchange chromatography on DEAE cellulose into 2 fractions, BmE DE1 and BmE DE2. BmE DE1 was marginally superior to whole BmmfES and BmE DE2 in detecting filarial IgG antibodies in human sera. 230 human sera from different groups of patients were screened against BmE DE1, which detected specific IgG in 83% of sera from microfilaraemic donors, 83% of sera from patients with clinical filariasis, 17% of sera from normal residents of an endemic area, and in none of the sera from persons living in a non-endemic area. The assay system using BmE DE1, with a sensitivity of 83% and specificity of 83%, should be very useful in detecting microfilaraemia, particularly in active clinical infections where the parasite is usually not seen.
由于无法获得足够的班氏吴策线虫寄生虫材料，因此对来自马来布鲁线虫的一种异源性丝虫抗原来进行班氏丝虫病的诊断研究。马来布鲁线虫微丝蚴排泄/分泌抗原（BmmfES）有效地抑制了微丝蚴血症病例的循环丝虫抗原组分（CFA2-1、9、11和12）以及班氏吴策线虫微丝蚴排泄/分泌抗原与抗丝虫血清免疫球蛋白的免疫球蛋白G（IgG）组分的结合。BmmfES通过在DEAE纤维素上进行离子交换色谱分离为两个组分，即BmE DE1和BmE DE2。在检测人血清中的丝虫IgG抗体方面，BmE DE1略优于完整的BmmfES和BmE DE2。针对BmE DE1对来自不同患者组的230份人血清进行筛查，其在83%的微丝蚴血症供血者血清、83%的临床丝虫病患者血清、17%的流行区正常居民血清中检测到特异性IgG，而在非流行区居民的血清中均未检测到。使用BmE DE1的检测系统，灵敏度为83%，特异性为83%，在检测微丝蚴血症方面应该非常有用，特别是在通常看不到寄生虫的活动性临床感染中。
