Dreyer G, Santos A, Norões J, Rocha A, Addiss D
Departamento de Parasitologia, Cidade Universitaria, Recife, PE, Brazil.
Trans R Soc Trop Med Hyg. 1996 May-Jun;90(3):288-9. doi: 10.1016/s0035-9203(96)90253-9.
To determine the extent to which Wuchereria bancrofti infections can be detected in asymptomatic amicrofilaraemic men in Greater Recife, Brazil, we studied 100 asymptomatic men who were long-term residents (> or = 15 years) of this filariasis-endemic area and who were amicrofilaraemic in 60 microL of capillary blood collected at night. Increasing amounts (1, 5, and 10 mL) of venous blood were collected in consecutive weeks, filtered, and examined for microfilariae; 27 men were eventually found to be microfilariaemic and 10 remained amicrofilaraemic but were found to be carriers of living adult W. bancrofti by ultrasound examination of the scrotal area. Thus, 37% of 'amicrofilaraemic' men (in 60 microL of blood) were found to be infected by more thorough investigation. Ultrasound is a valuable tool to identify adult worm infections in amicrofilaraemic persons, particularly for evaluation of serological assays and immunological studies in which the distinction between 'amicrofilaraemic adult worm carriers' and 'endemic normal subjects' is critical.
为了确定在巴西累西腓大都会区无症状无微丝蚴血症男性中能够检测到班氏吴策线虫感染的程度，我们研究了100名无症状男性，他们是这个丝虫病流行区的长期居民（≥15年），且夜间采集的60微升毛细血管血中无微丝蚴。连续数周采集递增剂量（1、5和10毫升）的静脉血，过滤后检查微丝蚴；最终发现27名男性有微丝蚴血症，10名仍无微丝蚴血症，但通过阴囊区域超声检查发现是活的成年班氏吴策线虫携带者。因此，通过更彻底的调查发现，37%的“无微丝蚴血症”男性（60微升血液中）受到感染。超声是识别无微丝蚴血症者成年虫感染的宝贵工具，尤其对于评估血清学检测和免疫学研究，其中区分“无微丝蚴血症成年虫携带者”和“流行区正常受试者”至关重要。
