Lin J, Lü H, Feng C
Arthritis Clinic and Research Center, People's Hospital, Beijing Medical University.
Chin Med J (Engl). 1996 Apr;109(4):313-6.
To examine the relationship between ankylosing spondylitis and heterogeneity of HLA-B27 in Chinese.
We subtyped HLA-B27 in 76 patients with ankylosing spondylitis and 1 200 healthy Chinese of Han nationality. Polymerase chain reaction was designed to amplify specific regions of class I (exon 2 and 3). Amplified sequences were tested with eight sequence-specific oligonucleotides to distinguish all HLA-B27 subtypes.
The HLA-B27 was closely related to ankylosing spondylitis and at least four subtypes presented in Chinese patients. B2704 was the most common subtype, occurring in 54.8% and 50.85% respectively in HLA-B27 positive ankylosing spondylitis patients and healthy controls. The main HLA-B27 subtypes in the HLA-B27 positive patients distributed in similar proportion to those of HLA-B27 positive healthy controls (P < 0.1).
The prevalences of various subtypes of HLA-B27 vary in different races. HLA-B2704 is the main subtype in Chinese Han nationality. The HLA-B27 specific residues that are shared by the subtypes might be involved in the pathogenesis of ankylosing spondylitis.
研究中国强直性脊柱炎患者与HLA - B27基因多态性的关系。
对76例强直性脊柱炎患者及1200名中国汉族健康人进行HLA - B27基因分型。采用聚合酶链反应扩增Ⅰ类基因（外显子2和3）的特定区域。用8种序列特异性寡核苷酸对扩增产物进行检测，以区分所有HLA - B27亚型。
HLA - B27与强直性脊柱炎密切相关，中国患者中至少存在4种亚型。B2704是最常见的亚型，在HLA - B27阳性的强直性脊柱炎患者和健康对照中分别占54.8%和50.85%。HLA - B27阳性患者中主要的HLA - B27亚型分布比例与HLA - B27阳性健康对照相似（P < 0.1）。
HLA - B27各亚型在不同种族中的分布存在差异。HLA - B2704是中国汉族人群中的主要亚型。各亚型共有的HLA - B27特异性残基可能参与强直性脊柱炎的发病机制。
