Shih H C, Liu S C, Chang C P, Tschen J S, Chiu H Y, Liu H C, Chang J G
Department of Laboratory Medicine, China Medical College Hospital, Taichung, Taiwan.
Kaohsiung J Med Sci. 2001 Oct;17(10):509-16.
The study was undertaken to determine the effects of HLA-B27 subtypes on susceptibility to ankylosing spondylitis (AS) in Taiwan Chinese, a polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) method was developed for subtyping of HLA-B27. In this series, there are 62 patients with AS who were tested HLA-B27 positive serologically and 738 normal persons over the age of 65. Among the 738 normal controls, 42 (5.7%) were HLA-B27 positive. There were six (14.3%) homozygous for B2704, 18 (42.9%) heterozygous for B2704, 2 (4.8%) double heterozygous for B2704 and B2705, one (2.3%) double heterozygous for B2704 and B2706, 2 (4.8%) homozygous for B2705, 11 (26.1%) heterozygous for B2705, and 2 (4.8%) heterozygous for B2706. In our patients with AS, 37 (59.7%) were homozygous for B2704 and 25 (40.3%) were heterozygous for B2704. The HLA-B27 carrier rate in Taiwan healthy old persons is estimated at 5.7%. Susceptibility to AS is determined by homozygosity for B2704. However, B2705 may be an indicator of protection against AS in Taiwan Chinese.
本研究旨在确定HLA - B27亚型对台湾华裔强直性脊柱炎（AS）易感性的影响，为此开发了一种聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）方法用于HLA - B27的亚型分型。在本研究系列中，有62例血清学检测HLA - B27阳性的AS患者以及738名65岁以上的正常人。在738名正常对照中，42人（5.7%）HLA - B27阳性。其中，6人（14.3%）为B2704纯合子，18人（42.9%）为B2704杂合子，2人（4.8%）为B2704和B2705双杂合子，1人（2.3%）为B2704和B2706双杂合子，2人（4.8%）为B2705纯合子，11人（26.1%）为B2705杂合子，2人（4.8%）为B2706杂合子。在我们的AS患者中，37人（59.7%）为B2704纯合子，25人（40.3%）为B2704杂合子。台湾健康老年人中HLA - B27携带率估计为5.7%。AS易感性由B2704纯合性决定。然而，B2705可能是台湾华裔人群中预防AS的一个指标。
