Guo W, Ren X, Feng C
Bone Tumor Research Laboratory, People's Hospital, Beijing Medical University.
Chin Med J (Engl). 1996 Feb;109(2):134-7.
Giant cell tumor of bone (GCT) is a comparatively common bone tumor in Chinese. Though GCT is generally considered as a benign tumor, it can pursue an aggressive course. The present study attempts to clarify whether the flow cytometric study can be an useful tool for prognosis of GCT patients.
DNA content and cell cycle studies on 96 pathologically confirmed GCT specimens and anti-GCT monoclonal antibody (GCF-5 McAb) labeling study on 30 GCTs were made by flow cytometry.
29 GCTs (30.2%) exhibited aneuploid and 67 (69.8%) diploid. Of 71 primary tumors, 16 (22.5%) exhibited aneuploid. The DNA index was 0.97+0.15, and the average S, G2/M phase cells 15.23+6.15%. Of 25 recurrent tumors, 13 (52%) were aneuploid, with an average DI of 1.08+0.17. The average S, G2/M phase cells was 19.56+7.12%. Between the primary and recurrent tumor groups, there was significant statistical difference both in S, G2/M phase cell percentage (P < 0.05) and DNA content (P < 0.01). There was no significant difference between Jaffe's grade I and grade II groups in DNA content or in S, G2/M phase cell percentage. GCF-5 McAb could combine with almost all cells of S, G2/M phase and part of the cells in G0/G1 phase. Significant relationship existed (r=0.468, P < 0.05) between the percentages of S, G2/M phase cells and GCF-5 positive cells. Between the primary and the recurrent tumor groups, there was a significant difference in the percentage of both S, G2/M phase cells and GCF-5 positive cells.
Apparently flow cytometric study is of value in the evaluation of the biological behavior of GCT as well as the prognosis of the patients.
骨巨细胞瘤（GCT）在中国是一种相对常见的骨肿瘤。尽管GCT通常被认为是良性肿瘤，但它可能会呈侵袭性病程。本研究试图阐明流式细胞术研究是否可成为评估GCT患者预后的有用工具。
采用流式细胞术对96例经病理证实的GCT标本进行DNA含量和细胞周期研究，并对30例GCT进行抗GCT单克隆抗体（GCF-5 McAb）标记研究。
29例GCT（30.2%）表现为非整倍体，67例（69.8%）为二倍体。在71例原发性肿瘤中，16例（22.5%）表现为非整倍体。DNA指数为0.97±0.15，S期、G2/M期细胞平均为15.23±6.15%。在25例复发性肿瘤中，13例（52%）为非整倍体，平均DI为1.08±0.17。S期、G2/M期细胞平均为19.56±7.12%。原发性肿瘤组和复发性肿瘤组之间，S期、G2/M期细胞百分比（P<0.05）和DNA含量（P<0.01）均存在显著统计学差异。Jaffe I级和II级组之间在DNA含量或S期、G2/M期细胞百分比方面无显著差异。GCF-5 McAb可与几乎所有S期、G2/M期细胞以及部分G0/G1期细胞结合。S期、G2/M期细胞百分比与GCF-5阳性细胞百分比之间存在显著相关性（r=0.468，P<0.05）。原发性肿瘤组和复发性肿瘤组之间，S期、G2/M期细胞百分比和GCF-5阳性细胞百分比均存在显著差异。
显然，流式细胞术研究在评估GCT的生物学行为以及患者预后方面具有价值。
