[Inducing effect of dimethy-4, 4'-dimethoxy-5, 6,5',6-dimethylenedioxybipheny-2, 2'-dicarboxylate (DDB) on differentiation of leukemia HL-60 cells].

OBJECTIVE

To study the effects of anti-hepatitis drug, DDB, on leukemia cell line HL-60.

METHOD

Cytobiological methods were used.

RESULT

DDB was inhibited the proliferation of HL-60 cells. About 50% of HL-60 cells treated with DDB (10(-4)mol/L) for 6 days exhibited NBT reduction, and phagocytosis activity was also enhanced by DDB (10(-4)mol/L) for 4 days. The HL-60 cells treated with DDB turned out to be mature granulocytes morphologically.

CONCLUSION

The activity of acid phosphatase in DDB-treated HL-60 cells was significantly increased. DDB can induce HL-60 cells to differentiate along granulocyte lineage.