Geng J, Lu J, Pan C
Department of Endocrinology, Chinese PLA General Hospital, Beijing.
Zhonghua Yi Xue Za Zhi. 1996 Jan;76(1):41-4.
To observe the effects of insulin and captopril on growth of mesangial cells and on production of interleukin-6 (IL-6) and tumor necrosis factor (TNF) in culture media of glomerular cells.
Streptozocin induced diabetic rats (DM rats) were compared with normal wistar rats.
The CPM value of 3H-TdR incorporated into mesangial cells in DM rats was significantly higher than that in Wistar rats. When the culture media was added by 5 x 10(-4) U/ml, 5 x 10(-3) U/ml and 5 x 10(-1) U/ml of insulin, the CPM value incorporated into mesangial cells and the concentration of IL-6 in the culture media of both DM and wistar rats were significantly higher than those of the control (no insulin). When captopril (10(-6) mol/L, 10(-4) mol/L, 10(-3) mol/L) was added into the media in different concentration, the cpm value of 3H-TdR and the concentration of TNF in DM rats were significantly lower than those of the control (no captopril). But the CPM value of 3H-TdR in Wistar rats was lower than that in the control, which was only seen in the concentration of 10(-3)M captopril.
Ins could promote 3H-TdR in corporation and increase the production of IL-6, and captopril inhibited 3H-TdR in corporation and decrease the production of TNF.
观察胰岛素和卡托普利对系膜细胞生长及对肾小球细胞培养基中白细胞介素 -6（IL-6）和肿瘤坏死因子（TNF）产生的影响。
将链脲佐菌素诱导的糖尿病大鼠（DM大鼠）与正常Wistar大鼠进行比较。
DM大鼠系膜细胞中掺入的3H-TdR的CPM值显著高于Wistar大鼠。当培养基中加入5×10⁻⁴U/ml、5×10⁻³U/ml和5×10⁻¹U/ml胰岛素时，DM大鼠和Wistar大鼠系膜细胞中掺入的CPM值以及培养基中IL-6的浓度均显著高于对照组（无胰岛素）。当以不同浓度（10⁻⁶mol/L、10⁻⁴mol/L、10⁻³mol/L）向培养基中加入卡托普利时，DM大鼠中3H-TdR的cpm值和TNF的浓度显著低于对照组（无卡托普利）。但仅在10⁻³M卡托普利浓度下，Wistar大鼠中3H-TdR的CPM值低于对照组。
胰岛素可促进3H-TdR掺入并增加IL-6的产生，而卡托普利抑制3H-TdR掺入并降低TNF的产生。
