Delannoy A S, Hober D, Bouzidi A, Lobert P E, Wattré P
Laboratoire de Virologie, CHRU, bâtiment IRFPPS, Lille, France.
Pathol Biol (Paris). 1996 May;44(5):461-3.
Monocyte/macrophage cell types can be infected with Human Cytomegalovirus (HCMV) could be a reservoir and a vehicle for virus spread in infected patients. We developed a model to study the effects of antiviral molecules on these cells. The monocytic-like cell line THP-1 and the human diploïd cells MRC-5 were used. THP-1 cells were cultivated with a phorbol 12-myristate 13-acetate (PMA) for 24h prior to the infection. We studied infection of these cells with reference strain (AD-169). A cell free virus suspension of HCMV was preincubated with hyperimmune polyvalent immunoglobulins. The infection of the cells was studied on the basis of immune detection of viral immediate early antigens (IEA) in nucleus 24h after culture. Our results showed that hyperimmune polyvalent immunoglobulins have been able to neutralize fibroblasts and THP-1 cells infection, whereas control antibodies did not inhibit the infection of the cells. This is the first report of the use of monocytic-like cells for testing the efficiency of anti-CMV molecules.
单核细胞/巨噬细胞类型可被人巨细胞病毒（HCMV）感染，可能是病毒在感染患者体内的储存库和传播载体。我们建立了一个模型来研究抗病毒分子对这些细胞的影响。使用了单核细胞样细胞系THP-1和人二倍体细胞MRC-5。在感染前，THP-1细胞用佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）培养24小时。我们用参考毒株（AD-169）研究了这些细胞的感染情况。将HCMV的无细胞病毒悬液与超免疫多价免疫球蛋白预孵育。在培养24小时后，基于对细胞核中病毒即刻早期抗原（IEA）的免疫检测来研究细胞的感染情况。我们的结果表明，超免疫多价免疫球蛋白能够中和成纤维细胞和THP-1细胞的感染，而对照抗体则不能抑制细胞的感染。这是首次使用单核细胞样细胞来测试抗CMV分子效率的报告。
