从蝙蝠分离出的裂锥虫亚属锥虫的细胞表面分析。

Cell surface analysis of trypanosomes of the subgenus Schizotrypanum isolated from bats.

作者信息

Pinto A S, Teixeira L F, Souto-Padrón T, Andrade A F

机构信息

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais 31270-010, Belo Horizonte, Brasil.

出版信息

Parasite. 1996 Jun;3(2):143-6. doi: 10.1051/parasite/1996032143.

DOI:10.1051/parasite/1996032143

PMID:8758552

Abstract

Two stocks (M5, M29) of trypanosomes of the subgenus Schizotrypanum were isolated from the bat Phyllostomus hastatus and analyzed for cell electrophoretic mobility (EPM) and lectin binding surface sites. Epimastigotes from the M5 and M29 stocks presented a mean EPM of around -0.57 and -0.56 microns. s-1.V-1.cm respectively. Differences in the agglutination profiles were detected between epimastigotes or trypomastigotes from the two parasite populations using lectins with specificity for D-GlcNAc, D-GalNAc, D-Gal and D-Man as probe. Major variation was observed between epimastigote forms. Additionally, the D-GlcNAc binding lectins WGA and BS II strongly interacted with the trypomastigote from both M5 and M29 stocks; this fact is evidence that these trypanosomes are distinct from Trypanosoma (Schizotrypanum) cruzi.

摘要

从蝙蝠长鼻蝠（Phyllostomus hastatus）中分离出裂锥虫亚属（Schizotrypanum）的两种锥虫株（M5、M29），并对其细胞电泳迁移率（EPM）和凝集素结合表面位点进行分析。来自M5和M29株的前鞭毛体的平均EPM分别约为-0.57和-0.56微米·秒⁻¹·伏⁻¹·厘米。以对D-GlcNAc、D-GalNAc、D-Gal和D-Man具有特异性的凝集素作为探针，检测到两个寄生虫群体的前鞭毛体或锥鞭毛体之间的凝集谱存在差异。在前鞭毛体形式之间观察到主要差异。此外，D-GlcNAc结合凝集素WGA和BS II与来自M5和M29株的锥鞭毛体强烈相互作用；这一事实证明这些锥虫与克氏锥虫（Trypanosoma (Schizotrypanum) cruzi）不同。