Teixeira L F, Gonçalves A M, Romanha A J, Steindel M, Pinto A S
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Parasitol Res. 1993;79(6):497-500. doi: 10.1007/BF00931591.
Two stocks and nine clones of trypanosomes of the subgenus Schizotrypanum were analyzed based on their electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzymes (zymodeme). The trypanosome stocks were isolated from Phyllostomus hastatus bats collected in different locations in Minas Gerais State, Brazil. The k-DNA pattern showed major variations between stocks and microvariations among the clones. Furthermore, in one of several cloning attempts, two different populations could be isolated. Among the six enzymes studied, glucose phosphate isomerase presented different patterns for stocks and clones. No similarity was found among bat trypanosomes and Trypanosoma (Schizotrypanum) cruzi standard zymodemes. This shows that bat trypanosomes are distinct from T. (S.) cruzi.
基于动质体DNA（k-DNA）小环的EcoR1消化产物（裂殖体）和同工酶（酶谱）的电泳图谱，对裂殖锥虫亚属的两种虫株和九个克隆的锥虫进行了分析。这些锥虫虫株是从巴西米纳斯吉拉斯州不同地点采集的长叶鼻蝠中分离出来的。k-DNA图谱显示虫株之间存在主要差异，克隆之间存在微小差异。此外，在几次克隆尝试中的一次中，分离出了两个不同的群体。在所研究的六种酶中，葡萄糖磷酸异构酶在虫株和克隆中呈现出不同的图谱。在蝙蝠锥虫和克氏锥虫（裂殖锥虫）标准酶谱之间未发现相似性。这表明蝙蝠锥虫与克氏锥虫不同。
