Cui M, Zheng P, Huang B
Second Clinical Hospital, Norman Bethune University of Medical Science, Changchun.
Zhonghua Fu Chan Ke Za Zhi. 1996 Mar;31(3):163-5.
To examine human papilloma virus (HPV) infection rate and types in several lesions of the genital tract and to observe the relation between HPV and host cells for differential handling of cases and for early diagnosis.
Tissues from 89 cases of condyloma acuminata, 76 cases of cervical cancer and swabs from 198 cases of normal vaginal were analysed by polymerase chain reaction for HPV infective rate. Main HPV types were compared and Southern blot was used to compare the physical pattern of HPV in condyloma acuminata and cervical cancer.
The positive rates of HPV-DNA in condyloma acuminata and cervical cancer were respectively 98.9% and 94.7%. The primary types of the former were HPV 6, 11 and of the latter mainly 16 and 18. The latent infective rates in normal vagina was 37.4%, among which, HPV 6, 11, 16, 18 consisted of only 21.6%. HPV11-DNA in condyloma acuminata existed mainly in episodic form. Most of HPV16-DNA in cervical cancer integrates into the host cells with variation.
The different types of HPV are related to particular morphology and characteristics of lesions. The relation of HPV-DNA to host cell and can offer some reference for the determination of the character of the lesion, and it may be award of malignant transformation.
检测生殖道几种病变中人类乳头瘤病毒（HPV）的感染率及型别，观察HPV与宿主细胞的关系，以便对病例进行鉴别处理及早期诊断。
采用聚合酶链反应分析89例尖锐湿疣、76例宫颈癌组织及198例正常阴道拭子的HPV感染率。比较主要HPV型别，并用Southern印迹法比较尖锐湿疣和宫颈癌中HPV的物理模式。
尖锐湿疣和宫颈癌中HPV-DNA阳性率分别为98.9%和94.7%。前者主要型别为HPV 6、11，后者主要为16、18。正常阴道潜伏感染率为37.4%，其中HPV 6、11、16、18仅占21.6%。尖锐湿疣中HPV11-DNA主要以游离形式存在。宫颈癌中多数HPV16-DNA整合入宿主细胞且发生变异。
不同类型的HPV与病变的特定形态和特征有关。HPV-DNA与宿主细胞的关系可为病变性质的判定提供参考，可能与恶性转化有关。
