Blumenthal R D, Stein R, Sharkey R M, Goldenberg D M, Ong G L, Klein K M, Mattes M J
Garden State Cancer Center at the Center for Molecular Medicine and Immunology, Newark, New Jersey 07103, USA.
Cancer Res. 1996 Aug 1;56(15):3508-15.
A new cell line derived from a human adenocarcinoma of the colon, GS-7, was propagated as a s.c. tumor in nude mice. This tumor histologically is a mucinous adenocarcinoma (also designated mucoid or colloid) with characteristic large mucin pools that are not lined by an epithelial layer but may contain scattered, randomly distributed cancer cells. Ten to 20% of human colorectal adenocarcinomas are of this histological type, but rapidly growing xenografts with this histology have been rarely used experimentally. This tumor, therefore, constitutes a useful model for similar human tumors. The mucin pools contain large amounts of carcinoembryonic antigen and tumor-associated glycoprotein 72, and the cells express epithelial glycoprotein 2 on their surface. The ability of antibodies injected i.v. to penetrate this tumor was investigated, using both biotinylated and radioiodinated antibodies (Abs). The results demonstrate that Abs can effectively penetrate the mucin pools, and that large amounts of Ab can localize there. This tumor type may have advantages as a target for certain forms of experimental immunotherapy.
一种源自人结肠腺癌的新细胞系GS - 7，在裸鼠体内作为皮下肿瘤进行传代培养。该肿瘤在组织学上是黏液腺癌（也称为黏液样或胶样癌），具有特征性的大黏液池，这些黏液池没有上皮层衬里，但可能含有散在的、随机分布的癌细胞。10%至20%的人结肠腺癌属于这种组织学类型，但具有这种组织学特征的快速生长的异种移植物很少用于实验。因此，这种肿瘤构成了类似人类肿瘤的有用模型。黏液池中含有大量癌胚抗原和肿瘤相关糖蛋白72，并且细胞表面表达上皮糖蛋白2。使用生物素化和放射性碘化抗体，研究了静脉注射抗体穿透这种肿瘤的能力。结果表明，抗体能够有效穿透黏液池，并且大量抗体能够在那里定位。这种肿瘤类型作为某些形式实验性免疫治疗的靶点可能具有优势。
