Ferre S, O'Connor W T, Svenningsson P, Bjorklund L, Lindberg J, Tinner B, Stromberg I, Goldstein M, Ogren S O, Ungerstedt U, Fredholm B B, Fuxe K
Division of Molecular and Cellular Neurochemistry, Department of Neuroscience, Karolinska Institute, S171 77 Stockholm Sweden.
Eur J Neurosci. 1996 Jul;8(7):1545-53. doi: 10.1111/j.1460-9568.1996.tb01617.x.
By using in vivo microdialysis it was found that one of the main functions of striatal dopamine D1 receptors is to selectively facilitate GABAergic neurotransmission in the 'direct' strioentopeduncular pathway. D1 receptors localized in the entopeduncular nucleus were also found to facilitate GABA release. However, results obtained from in vivo microdialysis, in vivo electrochemistry, immunohistochemistry and confocal laser microscopy suggested that entopeduncular D1 receptors could only be activated under pharmacological conditions. Adenosine A1 receptors were found to antagonistically modulate the D1-mediated regulation of the strioentopeduncular pathway. Furthermore, using in situ hybridization D1 and A1 receptors were shown to be colocalized in medium-sized striatal neurons. These results show that the strioentopeduncular neuron is a main locus for adenosine-dopamine interactions in the brain.
通过体内微透析发现，纹状体多巴胺D1受体的主要功能之一是选择性地促进“直接”纹状体-脚内核通路中的GABA能神经传递。还发现位于脚内核中的D1受体可促进GABA释放。然而，体内微透析、体内电化学、免疫组织化学和共聚焦激光显微镜检查的结果表明，脚内核D1受体仅在药理学条件下才能被激活。发现腺苷A1受体可拮抗调节纹状体-脚内核通路的D1介导作用。此外，通过原位杂交显示D1和A1受体共定位于中等大小的纹状体神经元中。这些结果表明，纹状体-脚内核神经元是大脑中腺苷-多巴胺相互作用的主要部位。
