核糖核酸酶P对基因N信使核糖核酸的切割会降低噬菌体λ的爆发量。
Cleavage by RNase P of gene N mRNA reduces bacteriophage lambda burst size.
作者信息
Li Y, Altman S
机构信息
Department of Biology, Yale University, New Haven, CT 06520, USA.
出版信息
Nucleic Acids Res. 1996 Mar 1;24(5):835-42. doi: 10.1093/nar/24.5.835.
Abstract
RNase P, an enzyme essential for tRNA biosynthesis, can be directed to cleave any RNA when the target RNA is in a complex with a short, complementary oligonucleotide called an external guide sequence (EGS). RNase P from Escherichia coli can cleave phage lambda N mRNA in vitro or in vivo when the mRNA is in a complex with an EGS. The EGS can either be separate from or covalently linked to M1 RNA, the catalytic RNA subunit of RNase P. The requirement for Mg2+ in the reaction in vitro is lower when the EGS is covalently linked to M1 RNA. Substrates made of DNA can also be cleaved by RNase P in vitro in complexes with RNA EGSs. When either kind of EGS construct is used in vivo, burst size of phage lambda is reduced by > or = 40%. Reduction in burst size depends on efficient expression of the EGS constructs. The product of phage lambda gene N appears to function in a stoichiometric fashion.
摘要
核糖核酸酶P是tRNA生物合成所必需的一种酶，当靶RNA与一种称为外部引导序列（EGS）的短互补寡核苷酸形成复合物时，它可被引导切割任何RNA。当噬菌体λ N mRNA与EGS形成复合物时，来自大肠杆菌的核糖核酸酶P可在体外或体内切割该mRNA。EGS既可以与核糖核酸酶P的催化性RNA亚基M1 RNA分开，也可以与之共价连接。当EGS与M1 RNA共价连接时，体外反应中对Mg2+的需求较低。由DNA构成的底物在体外与RNA EGS形成复合物时也可被核糖核酸酶P切割。当任何一种EGS构建体在体内使用时，噬菌体λ的爆发量减少≥40%。爆发量的减少取决于EGS构建体的有效表达。噬菌体λ基因N的产物似乎以化学计量方式发挥作用。
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