Chevalier B, Anglade P, Derouet M, Mollé D, Simon J
Endocrinologie De La Croissance Et Du Métabolisme, Station De Recherches Avicoles, INRA, Nouzilly, France.
Comp Biochem Physiol B Biochem Mol Biol. 1996 May;114(1):19-26. doi: 10.1016/0305-0491(95)02118-3.
Ducks (Anatidae Family, Anseriform order) are divided in two genera: Pekin duck (Anasplatyrhynchos genus) and Muscovy duck (Cairina moschata genus) and differ for their number of liver insulin receptors (despite rather similar plasma insulin levels). The possibility that the presence of different endogenous insulins account for the difference in insulin receptor number between the two duck species led us to purify, sequence and characterize the binding properties of Muscovy duck insulin. The sequence of Muscovy duck insulin (measured mass: 5729.11) was identical to that described in two other species from the Anseriforme order: Pekin duck or goose. The binding affinity of Muscovy duck insulin for rat liver insulin receptors (either membrane bound or solubilized receptors) was lower than that of porcine insulin (0.3), which most likely accounts for the low biological potency of Pekin duck insulin previously described. In contrast, liver receptors from chicken and both duck species exhibited the same affinity for duck and porcine insulin suggesting the presence of specific changes in the structure of binding sites of bird liver insulin receptors. The decrease in the number of insulin receptors in Muscovy duck liver is not therefore the consequence of a change at the level of the insulin molecule itself. As discussed, among bird insulins, the hypoactive "duck type" insulin would have appeared after the hyperactive "chicken type" insulin during the evolution of Aves.
鸭（雁形目鸭科）分为两个属：北京鸭（绿头鸭属）和番鸭（疣鼻栖鸭属），它们肝脏胰岛素受体数量不同（尽管血浆胰岛素水平相当相似）。两种鸭胰岛素受体数量差异可能是由于不同内源性胰岛素的存在，这促使我们对番鸭胰岛素进行纯化、测序并表征其结合特性。番鸭胰岛素的序列（测量质量：5729.11）与雁形目中另外两个物种：北京鸭或鹅中描述的序列相同。番鸭胰岛素对大鼠肝脏胰岛素受体（膜结合受体或可溶性受体）的结合亲和力低于猪胰岛素（0.3），这很可能是先前描述的北京鸭胰岛素生物活性低的原因。相比之下，鸡和两种鸭的肝脏受体对鸭胰岛素和猪胰岛素表现出相同的亲和力，这表明鸟类肝脏胰岛素受体结合位点结构存在特定变化。因此，番鸭肝脏中胰岛素受体数量的减少并非胰岛素分子本身水平变化的结果。如前所述，在鸟类胰岛素中，活性较低的“鸭型”胰岛素可能是在鸟类进化过程中活性较高的“鸡型”胰岛素之后出现的。
