Masuda I, Matsuo T, Yasuda T, Matsuo N
Department of Ophthalmology, Okayama University Medical School, Japan.
Invest Ophthalmol Vis Sci. 1996 Aug;37(9):1914-20.
To determine whether a reporter gene carried by liposomes can be introduced into the ocular tissues in vivo by different routes of administration.
Three different kinds of liposomes carrying plasmid DNA with beta-galactosidase gene were applied topically to the eye or were injected into the anterior chamber, subretinal space, and vitreous of adult Wistar rats. Gene expression was detected by enzymatic color reaction using X-gal as a substrate in enucleated eyes 1 day, 1 week, and 1 month after topical application or injection.
Topical application could transfer the gene to retinal ganglion cells. Injection into the anterior chamber delivered the gene to the basal layer of the corneal epithelium, ciliary epithelium, stroma of the ciliary body and iris, and retinal ganglion cells. Injection into the vitreous or subretinal space resulted in the expression of the gene in the ciliary epithelium, stroma of the ciliary body and iris, retinal ganglion cells, and retinal pigment epithelial cells.
Efficient and stable transfer of the functional gene could be achieved by liposomes in the cornea, iris, ciliary body, and retina of rats. Liposomes appear to be a promising vehicle for delivering therapeutic genes in vivo to mammalian intraocular tissues.
确定脂质体携带的报告基因能否通过不同给药途径在体内导入眼组织。
将三种携带含β-半乳糖苷酶基因质粒DNA的不同脂质体局部应用于成年Wistar大鼠的眼部，或注入前房、视网膜下间隙和玻璃体。在局部应用或注射后1天、1周和1个月，使用X-gal作为底物通过酶促显色反应在摘除的眼中检测基因表达。
局部应用可将基因转移至视网膜神经节细胞。注入前房可将基因递送至角膜上皮基底层、睫状体上皮、睫状体和虹膜基质以及视网膜神经节细胞。注入玻璃体或视网膜下间隙可使基因在睫状体上皮、睫状体和虹膜基质、视网膜神经节细胞以及视网膜色素上皮细胞中表达。
脂质体可在大鼠角膜、虹膜、睫状体和视网膜中实现功能基因的高效稳定转移。脂质体似乎是一种有前景的载体，可在体内将治疗性基因递送至哺乳动物眼内组织。
