Villacorta I, de Graaf D, Charlier G, Peeters J E
Laboratory of Parasitology, National Institute of Veterinary Research, Brussels, Belgium.
FEMS Microbiol Lett. 1996 Aug 15;142(1):129-32. doi: 10.1111/j.1574-6968.1996.tb08419.x.
Sporozoites of Cryptosporidium parvum excysted in vitro from bovine oocysts were incubated with monolayers of Madin-Darby bovine kidney cells. The extent of parasite colonisation was monitored by light microscopy and immunofluorescence. Electron microscopy confirmed the complete development and replication of C. parvum within Madin-Darby bovine kidney cells.
将从小牛卵囊中体外脱囊的微小隐孢子虫子孢子与麦迪逊-达比牛肾细胞单层进行孵育。通过光学显微镜和免疫荧光监测寄生虫的定殖程度。电子显微镜证实微小隐孢子虫在麦迪逊-达比牛肾细胞内完全发育并复制。
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