大肠杆菌DNA修复基因radA和sms是同一个基因。
Escherichia coli DNA repair genes radA and sms are the same gene.
作者信息
Song Y, Sargentini N J
机构信息
Science Division, Truman State University, Kirksville, Missouri, USA.
出版信息
J Bacteriol. 1996 Aug;178(16):5045-8. doi: 10.1128/jb.178.16.5045-5048.1996.
Abstract
Escherichia coli strains carrying radA100 or sms mutations were identical in their sensitivities to either methyl methanesulfonate or UV radiation treatment and in their plasmid complementation patterns for UV radiation survival. DNA sequencing analysis of the radA mutant and radA+ strains and comparison of their sequences with the published sms gene sequence showed the radA mutant to differ only by a G-to-A transition mutation, which is predicted to change a cysteine in a zinc-finger motif to tyrosine. The sms gene is concluded to be identical to the previously described radA gene.
摘要
携带radA100或sms突变的大肠杆菌菌株对甲磺酸甲酯或紫外线辐射处理的敏感性以及紫外线辐射存活的质粒互补模式相同。对radA突变体和radA+菌株进行DNA测序分析，并将它们的序列与已发表的sms基因序列进行比较，结果表明radA突变体仅因一个G到A的转换突变而有所不同，预计该突变会将锌指基序中的一个半胱氨酸变为酪氨酸。得出结论，sms基因与先前描述的radA基因相同。
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