大肠杆菌DNA修复基因radA和sms是同一个基因。
Escherichia coli DNA repair genes radA and sms are the same gene.
作者信息
Song Y, Sargentini N J
机构信息
Science Division, Truman State University, Kirksville, Missouri, USA.
出版信息
J Bacteriol. 1996 Aug;178(16):5045-8. doi: 10.1128/jb.178.16.5045-5048.1996.
Abstract
Escherichia coli strains carrying radA100 or sms mutations were identical in their sensitivities to either methyl methanesulfonate or UV radiation treatment and in their plasmid complementation patterns for UV radiation survival. DNA sequencing analysis of the radA mutant and radA+ strains and comparison of their sequences with the published sms gene sequence showed the radA mutant to differ only by a G-to-A transition mutation, which is predicted to change a cysteine in a zinc-finger motif to tyrosine. The sms gene is concluded to be identical to the previously described radA gene.
摘要
携带radA100或sms突变的大肠杆菌菌株对甲磺酸甲酯或紫外线辐射处理的敏感性以及紫外线辐射存活的质粒互补模式相同。对radA突变体和radA+菌株进行DNA测序分析，并将它们的序列与已发表的sms基因序列进行比较，结果表明radA突变体仅因一个G到A的转换突变而有所不同，预计该突变会将锌指基序中的一个半胱氨酸变为酪氨酸。得出结论，sms基因与先前描述的radA基因相同。
相似文献
1
Escherichia coli DNA repair genes radA and sms are the same gene.大肠杆菌DNA修复基因radA和sms是同一个基因。
J Bacteriol. 1996 Aug;178(16):5045-8. doi: 10.1128/jb.178.16.5045-5048.1996.
2
Role for radA/sms in recombination intermediate processing in Escherichia coli.RadA/Sms在大肠杆菌重组中间体加工中的作用。
J Bacteriol. 2002 Dec;184(24):6836-44. doi: 10.1128/JB.184.24.6836-6844.2002.
3
Molecular cloning and DNA sequencing of the radC gene of Escherichia coli K-12.大肠杆菌K-12 radC基因的分子克隆与DNA测序
Mutat Res. 1992 May;273(3):263-9. doi: 10.1016/0921-8777(92)90088-k.
4
Fine Epitope Mapping of Monoclonal Antibodies to the DNA Repair Protein, RadA.针对DNA修复蛋白RadA的单克隆抗体的精细表位图谱分析
Monoclon Antib Immunodiagn Immunother. 2017 Jun;36(3):83-94. doi: 10.1089/mab.2017.0021. Epub 2017 Jun 5.
5
Structural and functional characterization of the recR gene of Streptomyces.链霉菌recR基因的结构与功能特性
Mol Genet Genomics. 2001 Jun;265(4):663-72. doi: 10.1007/s004380100460.
6
Monoclonal antibodies against the Escherichia coli DNA repair protein RadA/Sms.针对大肠杆菌DNA修复蛋白RadA/Sms的单克隆抗体。
Hybridoma (Larchmt). 2012 Feb;31(1):25-31. doi: 10.1089/hyb.2011.0075.
7
Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair.大肠杆菌radD（yejH）基因：一种与辐射抗性和双链断裂修复有关的新功能。
Mol Microbiol. 2015 Mar;95(5):754-68. doi: 10.1111/mmi.12885. Epub 2015 Jan 16.
8
Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions.大肠杆菌RadA的遗传分析：功能基序与遗传相互作用
Mol Microbiol. 2015 Mar;95(5):769-79. doi: 10.1111/mmi.12899. Epub 2015 Jan 30.
9
Use of high and low level overexpression plasmids to test mutant alleles of the recF gene of Escherichia coli K-12 for partial activity.使用高水平和低水平过表达质粒来测试大肠杆菌K-12的recF基因突变等位基因的部分活性。
Genetics. 1993 Nov;135(3):643-54. doi: 10.1093/genetics/135.3.643.
10
Dominant negative mutator mutations in the mutS gene of Escherichia coli.大肠杆菌mutS基因中的显性负突变体突变
J Bacteriol. 1994 Sep;176(17):5393-400. doi: 10.1128/jb.176.17.5393-5400.1994.
引用本文的文献
1
Bacillus subtilis RecA interacts with and loads RadA/Sms to unwind recombination intermediates during natural chromosomal transformation.枯草芽孢杆菌 RecA 与 RadA/Sms 相互作用并加载 RadA/Sms，以在自然染色体转化过程中解旋重组中间体。
Nucleic Acids Res. 2019 Sep 26;47(17):9198-9215. doi: 10.1093/nar/gkz647.
2
The Lon protease-like domain in the bacterial RecA paralog RadA is required for DNA binding and repair.细菌RecA旁系同源蛋白RadA中的Lon蛋白酶样结构域对于DNA结合和修复是必需的。
J Biol Chem. 2017 Jun 9;292(23):9801-9814. doi: 10.1074/jbc.M116.770180. Epub 2017 Apr 21.
3
Replenishing the cyclic-di-AMP pool: regulation of diadenylate cyclase activity in bacteria.补充环二腺苷酸库：细菌中二腺苷酸环化酶活性的调控
Curr Genet. 2016 Nov;62(4):731-738. doi: 10.1007/s00294-016-0600-8. Epub 2016 Apr 13.
4
Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli.大肠杆菌中RecA的RadA/Sms旁系同源物介导的重组分支迁移
Elife. 2016 Feb 4;5:e10807. doi: 10.7554/eLife.10807.
5
Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions.大肠杆菌RadA的遗传分析：功能基序与遗传相互作用
Mol Microbiol. 2015 Mar;95(5):769-79. doi: 10.1111/mmi.12899. Epub 2015 Jan 30.
6
Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.辐射敏感基因 A (RadA) 靶向 DisA，即 DNA 完整性扫描蛋白 A，以负调控分枝杆菌中环状二腺苷酸合成酶的活性。
J Biol Chem. 2013 Aug 2;288(31):22426-36. doi: 10.1074/jbc.M113.464883. Epub 2013 Jun 10.
7
Proteomic and physiological responses of Kineococcus radiotolerans to copper.耐辐射球菌对铜的蛋白质组学和生理学响应。
PLoS One. 2010 Aug 26;5(8):e12427. doi: 10.1371/journal.pone.0012427.
8
RecA and RadA proteins of Brucella abortus do not perform overlapping protective DNA repair functions following oxidative burst.流产布鲁氏菌的RecA和RadA蛋白在氧化爆发后不执行重叠的保护性DNA修复功能。
J Bacteriol. 2006 Jul;188(14):5187-95. doi: 10.1128/JB.01994-05.
9
Host cell contact-induced transcription of the type IV fimbria gene cluster of Actinobacillus pleuropneumoniae.宿主细胞接触诱导胸膜肺炎放线杆菌IV型菌毛基因簇的转录。
Infect Immun. 2004 Feb;72(2):691-700. doi: 10.1128/IAI.72.2.691-700.2004.
10
Role for radA/sms in recombination intermediate processing in Escherichia coli.RadA/Sms在大肠杆菌重组中间体加工中的作用。
J Bacteriol. 2002 Dec;184(24):6836-44. doi: 10.1128/JB.184.24.6836-6844.2002.
本文引用的文献
1
THE PATHWAY AND CONTROL OF SERINE BIOSYNTHESIS IN ESCHERICHIA COLI.大肠杆菌中丝氨酸生物合成的途径与调控
J Biol Chem. 1963 Dec;238:3934-44.
2
Fpg protein of Escherichia coli is a zinc finger protein whose cysteine residues have a structural and/or functional role.大肠杆菌的Fpg蛋白是一种锌指蛋白，其半胱氨酸残基具有结构和/或功能作用。
J Biol Chem. 1993 Apr 25;268(12):9063-70.
3
4
Protein motifs 5. Zinc fingers.蛋白质基序5. 锌指结构
FASEB J. 1995 May;9(8):597-604.
5
DNA sequence analysis of gamma-radiation (anoxic)-induced and spontaneous lacId mutations in Escherichia coli K-12.大肠杆菌K-12中γ辐射（缺氧）诱导的和自发的lacId突变的DNA序列分析
Mutat Res. 1994 Sep 1;309(2):147-63. doi: 10.1016/0027-5107(94)90088-4.
6
A mutation (radA100) in Escherichia coli that selectively sensitizes cells grown in rich medium to x- or u.v.-radiation, or methyl methanesulphonate.大肠杆菌中的一种突变（radA100），它能选择性地使在丰富培养基中生长的细胞对X射线或紫外线辐射，或甲磺酸甲酯敏感。
Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Sep;42(3):339-46. doi: 10.1080/09553008214551251.
7
Characterization of a new radiation-sensitive mutant, Escherichia coli K-12 radC102.一种新型辐射敏感突变体——大肠杆菌K-12 radC102的特性分析
Radiat Res. 1984 Mar;97(3):615-25.
8
Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli.定量分析recA、recB、recC、recF、recJ、recN、lexA、radA、radB、uvrD和umuC基因在大肠杆菌修复X射线诱导的DNA双链断裂中的作用。
Radiat Res. 1986 Jul;107(1):58-72.
9
Ionizing and ultraviolet radiation-induced reversion of sequenced frameshift mutations in Escherichia coli: a new role for umuDC suggested by delayed photoreactivation.电离辐射和紫外线诱导大肠杆菌中测序的移码突变回复：延迟光复活提示umuDC的新作用
Mutat Res. 1987 Jul;179(1):55-63. doi: 10.1016/0027-5107(87)90041-8.
10
New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.在大肠杆菌中分离出各种RecA蛋白活性的新recA突变，为RecA蛋白在紫外线诱变中的额外作用提供了证据。
J Bacteriol. 1989 May;171(5):2415-23. doi: 10.1128/jb.171.5.2415-2423.1989.
Zotero 插件