Minami E, Kuchitsu K, He D Y, Kouchi H, Midoh N, Ohtsuki Y, Shibuya N
Department of Cell Biology, National Institute of Agrobiological Resources, Japan.
Plant Cell Physiol. 1996 Jun;37(4):563-7. doi: 10.1093/oxfordjournals.pcp.a028981.
By using subtracted probes, two cDNA clones of rice, EL2 and EL3, were isolated as genes responsive within 6 min to N-acetylchitoheptaose, a potent biotic elicitor for phytoalexin biosynthesis. Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes.
通过使用扣除探针,分离出了水稻的两个cDNA克隆EL2和EL3,它们是对N-乙酰七聚壳寡糖(一种用于植保素生物合成的强效生物激发子)在6分钟内产生响应的基因。对cDNA序列的分析表明,EL2和EL3都编码碱性蛋白,与已知基因的蛋白没有明显相似性。