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通过用N-乙酰基七聚壳聚糖(一种用于植保素生产的生物激发子)处理,在悬浮培养的水稻细胞中快速且短暂激活的两个新基因。

Two novel genes rapidly and transiently activated in suspension-cultured rice cells by treatment with N-acetylchitoheptaose, a biotic elicitor for phytoalexin production.

作者信息

Minami E, Kuchitsu K, He D Y, Kouchi H, Midoh N, Ohtsuki Y, Shibuya N

机构信息

Department of Cell Biology, National Institute of Agrobiological Resources, Japan.

出版信息

Plant Cell Physiol. 1996 Jun;37(4):563-7. doi: 10.1093/oxfordjournals.pcp.a028981.

Abstract

By using subtracted probes, two cDNA clones of rice, EL2 and EL3, were isolated as genes responsive within 6 min to N-acetylchitoheptaose, a potent biotic elicitor for phytoalexin biosynthesis. Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes.

摘要

通过使用扣除探针,分离出了水稻的两个cDNA克隆EL2和EL3,它们是对N-乙酰七聚壳寡糖(一种用于植保素生物合成的强效生物激发子)在6分钟内产生响应的基因。对cDNA序列的分析表明,EL2和EL3都编码碱性蛋白,与已知基因的蛋白没有明显相似性。

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