从用几丁质激发子处理的悬浮培养水稻细胞中克隆并鉴定编码内贝壳杉烯-12,15-二烯合酶（一种假定的二萜类植物抗毒素生物合成酶）的cDNA
Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.
作者信息
Cho Eun-Min, Okada Atsushi, Kenmoku Hiromichi, Otomo Kazuko, Toyomasu Tomonobu, Mitsuhashi Wataru, Sassa Takeshi, Yajima Arata, Yabuta Goro, Mori Kenji, Oikawa Hideaki, Toshima Hiroaki, Shibuya Naoto, Nojiri Hideaki, Omori Toshio, Nishiyama Makoto, Yamane Hisakazu
机构信息
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Plant J. 2004 Jan;37(1):1-8. doi: 10.1046/j.1365-313x.2003.01926.x.
We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice.
我们从用几丁质激发子处理的悬浮培养水稻细胞中分离并鉴定了一个编码新型二萜环化酶OsDTC1的cDNA。OsDTC1作为内贝壳杉烯-12,15-二烯合酶发挥作用，该酶被认为在最近作为水稻二萜类植保素分离出的(-)-植物抗毒素的生物合成中起关键作用。在紫外线（UV）照射的水稻叶片中也证实了OsDTC1 mRNA的表达。此外，我们鉴定出内贝壳杉烯-12,15-二烯，一种(-)-植物抗毒素的假定二萜烃前体，是几丁质激发的悬浮培养水稻细胞和UV照射的水稻叶片中的内源性化合物。这里分离出的OsDTC1 cDNA将是研究水稻中(-)-植物抗毒素生物合成调控机制的有用工具。
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