胰腺切除患者的血清样本含有胰蛋白酶原免疫反应性。

Serum samples from pancreatectomized patients contain trypsinogen immunoreactivity.

作者信息

Itkonen O, Stenman U H, Osman S, Koivunen E, Halila H, Schröder T

机构信息

Department of Clinical Chemistry, Helsinki University Central Hospital, Finland.

出版信息

J Lab Clin Med. 1996 Jul;128(1):98-102. doi: 10.1016/s0022-2143(96)90118-3.

DOI:10.1016/s0022-2143(96)90118-3

PMID:8759941

Abstract

The concentrations of trypsinogen-1 and -2 in serum samples from patients who have undergone pancreatectomy were measured by highly sensitive and specific time-resolved immunofluorometric assays. The isoenzyme pattern was determined by ion-exchange chromatography and determination of immunoreactivity in the fractions. All samples contained trypsinogen-2, the mean level being one fifth of that in healthy controls. Trypsinogen-1 was detected in one of nine samples. In addition to the main trypsinogen isoenzymes, we observed in normal serum two trypsinogen isoenzymes previously found in mucinous ovarian cyst fluid. Our results suggest that trypsinogen is not exclusively expressed by the pancreas and certain tumors but that it also may be produced by normal extrapancreatic tissues. This should be considered when an assay of trypsinogen in serum is used for clinical purposes.

摘要

通过高灵敏度和特异性的时间分辨免疫荧光分析法，测定了接受胰腺切除术患者血清样本中胰蛋白酶原-1和-2的浓度。通过离子交换色谱法和各组分免疫反应性测定来确定同工酶模式。所有样本均含有胰蛋白酶原-2，其平均水平为健康对照的五分之一。在九个样本中的一个检测到了胰蛋白酶原-1。除了主要的胰蛋白酶原同工酶外，我们在正常血清中还观察到了先前在黏液性卵巢囊肿液中发现的两种胰蛋白酶原同工酶。我们的结果表明，胰蛋白酶原并非仅由胰腺和某些肿瘤表达，正常胰腺外组织也可能产生。当将血清中胰蛋白酶原检测用于临床目的时应考虑到这一点。